Alkaline Phosphatase, Liver/Bone/Kidney (ALPL) BioAssay ELISA Kit (Mouse)

£1,007.00 96 Tests

Alkaline Phosphatase, Liver/Bone/Kidney (ALPL) BioAssay ELISA Kit (Mouse) is a sandwich enzyme immunoassay for in vitro quantitative measurement of ALPL in mouse serum, plasma, tissue homogenates and other biological fluids.

SKU: US023245 Categories: ,

Additional information

Detection Range

0.156-10ng/ml
Sensitivity

0.059ng/ml
Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%
Kit Components

*023245A: Microtiter Plate, 1×96 wells, Pre-coated; ready to use
*023245B: Standard, 2×1 vial
023245C: Standard Diluent, 1x20ml
*023245D: Detection Reagent A, 1x120ul
*023245E: Detection Reagent B, 1x120ul
023245F: Assay Diluent A, 1x12ml
023245G: Assay Diluent B, 1x12ml
023245H: TMB Substrate, 1x9ml
*023245J: Positive Control, 1×1 vial
023245K: Stop Solution, 1x6ml
023245L: Wash Buffer, 30X, 1x20ml
Precaution

The Stop Solution (023245K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability

Store *023245A, *023245B, *023245D, *023245E and *023245J at -20?C. After reconstitution of *023245J, store at 4?C and use within 5 days. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Notes on 023245J

Positive Control
Intended Use

The Positive Control is from a mouse serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.
Characteristics

The control vial contains mouse ALPL in mouse serum (lyophilized powder). Note: The mouse based serum used in this product is from a healthy specimen(s) of SPF standard (specific pathogen-free animals).
Expected Value

1.13ng/ml
Usage

Dissolve with 150ul standard dilutent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20?C. After reconstitution, store Positive Control vial at 4?C and use within 5 days.
Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37?C.
3. Add 100ul prepared Detection Reagent A. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.
Source Antigen

Mouse
Shelf Life

6 Months

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