Alpha-1-Microglobulin/Bikunin Precursor (a1M) BioAssay ELISA Kit (Mouse)

£949.00 96 Tests

Alpha-1-Microglobulin/Bikunin Precursor (a1M) BioAssay ELISA Kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of a1M in mouse serum, plasma and other biological fluids.

SKU: US023266 Categories: ,

Additional information

Detection Range

2.47-200ug/ml

Sensitivity

0.88ug/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components

*023266A: Microtiter Strips, 1×48 wells or 1×96 wells, Pre-coated, ready to use
*023266B: Standard, 2×1 vial
023266C: Standard Diluent, 1x10ml or 1x20ml
*023266D: Detection Reagent A (green), 1x60ul or 1x120ul
*023266E: Detection Reagent B (red), 1x60ul or 120ul
023266F: Assay Diluent A, 1x6ml or 1x12ml
023266G: Assay Diluent B, 1x6ml or 1x12ml
023266H: TMB Substrate, 1×4.5ml or 1x9ml
*023266J: Positive Control, 7.78ug/ml, 1x1vial
023266K: Stop Solution, 1x3ml or 1x6ml
023266L: Wash Buffer, 30X, 1x10ml or 1x20ml

Precaution

The Stop Solution (023266K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *023266A, *023266B, *023266D, *023266E and *023266J at -20?C. After reconstitution of *023266J, store at 4?C and use within 5 days. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Notes on J

Positive Control

Intended Use

The control vials are from mouse serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.

Characteristics

The control vials contain mouse a1M in mouse serum (lyophilized powder). Note: The mouse based material used in this product is from healthy mice of SPF standard (specific pathogen-free animals).

Expected Values

7.78ug/ml
The concentration of the control was determined by subtracting two standard deviations to the mean optical density value of twenty QC sample replicates and calculating the corresponding concentration.

Usage

Dissolve with 150ul standard dilutent and let stand for 10 minutes. Mix gently before use. In the experiment, add 50ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: After reconstitution, store Positive Control vial at 4?C and use within 5 days.

Assay Procedure Summary

1. Prepare all reagents, samples and standards;
2. Add 50ul standard or sample to each well.
3. Add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen

Mouse

Shelf Life

6 Months