BIOSPECIMENS

We are delighted to offer one of the most comprehensive ranges of biological specimens in Europe

We maintain a comprehensive inventory of normal and disease state human donor material, clinical samples, biofluids, matched sets and prospective collections.  All samples are collected under the appropriate IRB approval with appropriate donor consents in place.  We have collections in the USA, India and Europe.

Our animal material is collected in UK (unless stated) and harvested from animals in fully registered facilities under veterinary supervision from an extensive range of animal species and strains. Material is also available from a large range of agricultural species.

LSG animal-derived products are sourced from laboratory animals bred under controlled conditions. Collections are made from animals that have been fasted and which are drug naïve. This results in a high quality product with minimal batch variation and allows for complete traceability.

Serum: All serum, unless requested otherwise, is collected ‘off clot’ without additives or preservatives.

Complement Preserved Serum: Complement preserved serum and lyophilised complement products are also available.

Plasma and Whole Blood: This material is prepared with the anticoagulant of choice. Standard anticoagulants are:

        • ACD
        • Sodium EDTA
        • CTAD
        • Lithium Heparin
        • Sodium Citrate
        • Potassium EDTA (K2 or K3)
        • Sodium Heparin
        • Potassium Oxalate/Sodium Fluoride

PBMCs: PBMC extractions are available from a wide variety of animal species, prepared to individual protocols. These can be supplied fresh, as Buffy Coats or as frozen cell separations.

Processes and Presentations:

          • Medium and Heavy Charcoal stripping
          • Ultra filtration to specified molecular weight cut offs
          • Custom aliquoting

Material Specification Options:

            • Individual donor
            • Non filtered material
            • Pooled donors
            • Sterile filtered to 0.2 micron
            • Single gender or 0.45 micron
            • Pooled gender
            • Fibrinogen free
            • Specific strains
            • Ultra centrifugation

Tissues

These are collected to customer specification and may be supplied frozen or fresh.

Microsomes and S9 Fractions

LSG offers a large range of pooled microsomes and S9 fractions from liver and other tissues from a variety of animal species and breeds. Animal liver cytosol is also available. Please contact us for further information.

Homogenisation

LSG are pleased to offer homogenised fresh tissues from a variety of animal sources. Our UK facility is now able to offer uniform dilutions of tissue suspensions that have been homogenised using Wheaton Potter-Elvehjem Tissue Grinders.

Our standard homogenisation buffer is sterile saline containing gentamicin [0.8 μg/ml] and chloramphenicol [0.05mg/ml] however we are happy to prepare material to specific protocols. Protocols may be tailored to meet specific requirements for assay targets in various ways, including:

              • Addition of Protease inhibitors for protein activity and characterisation assays
              • Addition of surfactants/detergents for denatured protein use in immunoassays
              • Addition of Chaotropes for RNA
              • Addition of EDTA to prevent DNAase activity

Further testing available on request

In addition to a large range of blood products LSG can also supply tissues and bodily fluids. Presentations for whole tissues or sections include frozen, homogenised and formalin or paraffin fixed. Tissues may also be prepared to specific protocols and whole animal necroscopy is also offered.

Most of these items are prepared to order so please contact us to enquire as to the time frame for delivery. Please contact our Customer Services

Department to enquire about the availability of products that are not listed.

Normal human material is collected from stringently screened donors at FDA registered donor centres. The donors must test negative for RPR, HIV 1, HIV 2, Hepatitis B Surface Antigen, HCV and HIV 1-Ag using approved FDA methodology. The donors must have normal ALT levels. Qualifying donors are also subject to a physical examination and verbal screening.

Serum: All serum, unless requested otherwise, is collected ‘off clot’ without additives or preservatives

Complement Preserved serum: Complement preserved serum and lyophilised complement products are also available

Plasma and Whole Blood: This material is prepared with the anticoagulant of choice. Standard anticoagulants are:

        • ACD
        • CTAD
        • Sodium Citrate
        • Sodium Heparin
        • Sodium EDTA
        • Lithium Heparin
        • Potassium EDTA (K2 or K3)
        • Potassium Oxalate/Sodium Fluoride

    Processes and Presentations

      • Medium and Heavy Charcoal stripping
      • Ultra filtration to specified molecular weight cut offs
      • Custom aliquoting
      • Non filtered material and Sterile filtered to 0.2 micron or 0.45 micron
      • Fibrinogen free
      • Ultra centrifugation

Material Specification Options:

        • Individual donors
        • Pooled donors
        • Single gender
        • Pooled gender
        • Specific demographic groups
        • Age, race and gender specifications
        • Specific dietary requirements

Associated Products

If you would like further information, please call us on 01234 889180 or email sales@lifesciencegroup.co.uk.