Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) BioAssay ELISA Kit (Human)

£990.00 96 Tests

Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) BioAssay ELISA Kit (Human) is a competitive inhibition enzyme immunoassay for in vitro quantitative measurement of ADCYAP1 in human serum, plasma, tissue homogenates and other biological fluids.

SKU: US023137 Categories: ,

Additional information

Detection Range

6.17-500pg/ml
Sensitivity

2.62pg/ml
Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%
Kit Components

*023137A: Microtiter Strips, 1×96 wells
*023137B: Standard, 2×1 vial
023137C: Standard Diluent, 1x20ml
*023137D: Detection Reagent A, 1x120ul
*023137E: Detection Reagent B, 1x120ul
023137F: Assay Diluent A, 1x12ml
023137G: Assay Diluent B, 1x12ml
023137H: TMB Substrate, 1x9ml
023137K: Stop Solution, 1x6ml
023137L: Wash Buffer, 30X, 1x20ml
Precaution

The Stop Solution (023137K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Storage and Stability

Store *023137A, *023137B, *023137D and *023137E at -20?C. Store all the other components at 4?C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37?C.
3. Aspirate and wash 3 times.
4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
5. Aspirate and wash 5 times.
6. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37?C.
7. Add 50ul Stop Solution. Read at 450nm immediately.
Source Antigen

Human
Shelf Life

6 Months

Related products