a-Fetoprotein (aFP) BioAssay ELISA Kit (Rat)
£1,007.00 96 Tests
a-Fetoprotein (aFP) BioAssay ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of aFP in rat serum, plasma, tissue homogenates and other biological fluids.
Additional information
| Detection Range | 0.312-20ng/ml |
|---|---|
| Sensitivity | 0.122ng/ml |
| Precision | Intra-Assay CV: Inter-Assay CV: |
| Kit Components | *023294A: Microtiter Strips, 1×48 wells or 1×96 wells, Pre-coated, ready to use |
| Precaution | The Stop Solution (023294K) included for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. |
| Storage and Stability | Store *023294A, *023294B, *023294D, *023294E and *023294J at -20?C. After reconstitution of *023294J, store at 4?C and use within 5 days. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. |
| Notes on 023294J | Positive Control |
| Intended Use | The positive control is from a rat serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly. |
| Characteristics | The positive control vial contains rat aFP in rat serum (lyophilized powder). Note: The rat based serum used in this product are from healthy rats of SPF standard (specific pathogen-free animals). |
| Expected Values | 3.85ng/ml |
| Usage | Dissolve with 150ul standard diluent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20?C. After reconstitution, store Positive Control vial at 4?C and use within 5 days. |
| Assay Procedure Summary | 1. Prepare all reagents, samples and standards. |
| Shelf Life | 6 Months |
