Dr. Al Hubaysh is our in-house research scientist. Below she has shared with us some vital tips for cell culturing.

  1. Always Maintain Sterile Conditions: You must ensure aseptic technique throughout your cell culture process. Sterile equipment, proper handling, and regular cleaning of the workspace are essential to prevent contamination.
  2. Optimize Cell Density: Seed your cells at an appropriate density to avoid overcrowding or sparse growth. This helps maintain a healthy and robust cell population.
  3. Minimize the Passage Number: Limit the number of times cells are passaged to avoid replicative senescence and genetic drift. Higher-passage cells may behave differently than early-passage ones. Minimizing the passage number will increase the stability of cells and the quality of the results.
  4. Regularly Inspect Your Cells: Use a microscope to monitor cell morphology and health. Any irregularities or signs of contamination should be addressed promptly.
  5. Feed Cells Appropriately: Provide fresh medium to cells at the right intervals. Depleted nutrients can hinder cell growth while overfeeding might lead to excessive waste buildup.
  6. Remember to Keep Detailed Records: Maintain thorough and organized records of your cell culture protocols, including passage numbers, growth conditions, and any observed changes. This is important for reproducibility, repeatability, and traceability.
  7. Plan for Cryopreservation: Have a reliable cryopreservation protocol in place to preserve cell lines for future use. This can act as a backup and prevent the loss of valuable cell lines.
  8. Regularly Test for Mycoplasma: Mycoplasma contamination can be silent but detrimental. Routinely test your cultures to ensure they are free from mycoplasma contamination.

Remember that successful cell culture requires patience, attention to detail, and a commitment to maintaining optimal conditions for your cells’ growth and health.