Viral Sample Collection Buffers and Kits

Sample Transport Kits

Life Science Group Ltd (LSG) can offer the following range of sample collection kits suitable for the collection of swab samples for a variety of assay purposes.  These kits have all been validated for suitability to collect samples for testing for Covid-19 infection and may be used for a wide range of viruses and other infectious organisms.

LSG Viral Transport Medium (VTM) is a non-propagating transport culture medium intended for transport of clinical material from the collection site to the laboratory for testing. This medium is designed to sustain the viability of viruses, chlamydia and mycoplasma for research, diagnostic tests, molecular biology techniques and conventional cell culture methods and may also be used for the short term storage of samples.

LSG VTM ensures proper collection, transport and storage of clinical specimens containing upper respiratory viruses, in particular of SARS-CoV-2, the virus responsible for COVID-19 . The medium is formulated to preserve genome (RNA) integrity of the virus while suppressing growth of bacteria and fungi that may be present in clinical samples from the human respiratory system.

The constituents are designed to provide an isotonic solution containing protective protein, antibiotics to control microbial contamination, and one or more buffers to control the pH.

Liquid transport media are used primarily for transporting swabs or materials released into the medium from a collection swab. Liquid media may be added to the specimens when inactivation of the viral agent is likely and when the resultant dilution is acceptable.  This Viral Transport Medium (CDC) is manufactured to current Center for Disease Control and Prevention (CDC) protocol SOP SOP#: DSR-052-01.

This product is a sampling transport medium for the collection of specimens such as saliva, nasal swab and pharyngeal swabs. The preservation solution uses a PBS buffer with the addition of  foetal bovine serum (FBS) and antibiotics. This buffer offers good sample preservation, prevents bacterial and fungal contamination and can maintain the activity of the virus for nucleic acid detection, virus isolation and other purposes.  This product is also available in bulk format if required.  The LSG VTM is NOT an inactivating medium.

Applications:

For the collection of:

  • virus samples (COVID-19, Respiratory Syncytial Virus, Influenza A and B, Herpes Simplex I and II, Cytomegalovirus, Echovirus, etc.)
  • Chlamydia tachomatis and pneumoniae
  • Mycoplasma hominis and pneumonia
  • Ureaplasma urealyticum
  • Enzyme Immuno Assays (EIA)

Product code     Description

KIT-VTA-001G-N

LSG Sample Collection Kit  with  Viral Transport Medium 1mL in 12mL  with swab in a pouch

Kit

KIT-VTA-001G-O

LSG Sample Collection Kit  with  Viral Transport Medium 1mL in 7mL  with swab in a pouch

Kit

KIT-VTA-001U-N

LSG Sample Collection Kit  with  Viral Transport Medium 3mL in 12mL  with swab in a pouch

Kit

KIT-VTA-001U-O

LSG Sample Collection Kit  with  Viral Transport Medium 3mL in 7mL  with swab in a pouch

Kit

KIT-VTA-001X-N

LSG Sample Collection Kit  with  Viral Transport Medium 2mL in 12mL  with swab in a pouch

Kit

KIT-VTA-001X-O

LSG Sample Collection Kit  with  Viral Transport Medium 2mL in 7mL  with swab in a pouch

Kit

The Inactivir® range of Viral Inactivation Buffers have been developed to be used in sample tubes to inactivate viral pathogens that are present in patient or other potentially pathogenic samples.

The inactivation of viral pathogens on sample collection using Inactivir® will facilitate risk assessments that educe the requirement for Category 2+/3 handling of potentially pathogenic samples, increasing both the safety of workers and the speed and ease of testing.

These buffers have been shown to offer stability of RNA in samples for an extended time period of up to 5 days at ambient temperatures (21°C) and up to 10 weeks when stored at -20°C.  These chaotrope containing Inactivir™ Viral Inactivation Buffers are highly effective at preserving RNA in samples for subsequent analysis.

Inactivir® PLUS is again a guanidine-based buffer that uses an alternate REACH-compliant detergent. Under REACH regulations Triton X-100 should not be used from 2021 onwards and is only in current large-scale use due to pandemic exclusions. This buffer is REACH compliant and features an improved buffer system for stabilisation of RNA in samples. The Inactivir PLUS kit is suitable for the collection of virus specimens, such as saliva, nasal swab and pharyngeal samples, demonstrating complete viral inactivation of SARS nCoV-19, Ebola, Influenza A & B, RSV and a wide range of other viruses.  The preservation solution protects the viral nucleic acid from degradation for one week at room temperature (15 ~ 25°C). Inactivir PLUS is compatible with many lysis buffers in DNA/RNA extraction kits.

This product is also available in bulk format if required.

Product code     Description

KIT-VIB-002G-N

LSG Sample Collection Kit with Inactivir PLUS Viral Inactivation Buffer 1mL in 12mL  with swab in a pouch

Kit

KIT-VIB-002G-O

LSG Sample Collection Kit  with Inactivir PLUS Viral Inactivation Buffer 1mL in 7mL  with swab in a pouch

Kit

KIT-VIB-002U-N

LSG Sample Collection Kit  with Inactivir PLUS Viral Inactivation Buffer 3mL in 12mL  with swab in a pouch

Kit

KIT-VIB-002U-O

LSG Sample Collection Kit  with Inactivir PLUS Viral Inactivation Buffer 3mL in 7mL  with swab in a pouch

Kit

KIT-VIB-002X-N

LSG Sample Collection Kit  with Inactivir PLUS Viral Inactivation Buffer 2mL in 12mL  with swab in a pouch

Kit

KIT-VIB-002X-O

LSG Sample Collection Kit  with Inactivir PLUS Viral Inactivation Buffer 2mL in 7mL  with swab in a pouch

Kit

Inactivir® SD LITE is a non-guanidine buffer that is based on established inactivation principles used in production of human products for several decades with no evidence of viral transmission through those products in the entire timescale. Specific testing has shown inactivation of a wide range of viruses including SARS nCov-19. This buffer is designed for Point of Care (POC) or near-POC testing, with minimal additional components that could interfere with downstream testing. It does not stabilise RNA within samples and is suited for immediate testing use.  This buffer is used to rapidly release nucleic acids from nasopharyngeal swabs, oropharyngeal swabs and saliva samples. After nucleic acid is released, it can be directly used as a template for nucleic acid amplification, such as PCR, qPCR, RT-qPCR and isothermal amplification without nucleic acid purification. The lysis material contained in the storage solution in the sample tube can inactivate the virus quickly and preserve the RNA. 

This product is also available in bulk format if required.

Product code     Description

KIT-VIB-003G-N

LSG Sample Collection Kit  with Inactivir SD LITE Viral Inactivation Buffer 1mL in 12mL  with swab in a pouch

Kit

KIT-VIB-003G-O

LSG Sample Collection Kit  with Inactivir SD LITE Viral Inactivation Buffer 1mL in 7mL  with swab in a pouch

Kit

KIT-VIB-003U-N

LSG Sample Collection Kit  with Inactivir SD LITE Viral Inactivation Buffer 3mL in 12mL  with swab in a pouch

Kit

KIT-VIB-003U-O

LSG Sample Collection Kit  with Inactivir SD LITE Viral Inactivation Buffer 3mL in 7mL  with swab in a pouch

Kit

KIT-VIB-003X-N

LSG Sample Collection Kit  with Inactivir SD LITE Viral Inactivation Buffer 2mL in 12mL  with swab in a pouch

Kit

KIT-VIB-003X-O

LSG Sample Collection Kit  with Inactivir SD LITE Viral Inactivation Buffer 2mL in 7mL  with swab in a pouch

Kit

Inactivir® SD COMPLETE has the same non-guanidine inactivation system as Inactivir SD LITE, but additionally contains buffer components that inactivate nucleotide degrading enzymes including RNAses. It therefore stabilizes samples to allow remote testing away from the site of sampling.

This product is also available in bulk format if required.

Product code     Description

KIT-VIB-004G-N

LSG Sample Collection Kit  with Inactivir SD COMPLETE Viral Inactivation Buffer 1mL in 12mL  with swab in a pouch

Kit

KIT-VIB-004G-O

LSG Sample Collection Kit  with Inactivir SD COMPLETE Viral Inactivation Buffer 1mL in 7mL  with swab in a pouch

Kit

KIT-VIB-004U-N

LSG Sample Collection Kit  with Inactivir SD COMPLETE Viral Inactivation Buffer 3mL in 12mL  with swab in a pouch

Kit

KIT-VIB-004U-O

LSG Sample Collection Kit  with Inactivir SD COMPLETE Viral Inactivation Buffer 3mL in 7mL  with swab in a pouch

Kit

KIT-VIB-004X-N

LSG Sample Collection Kit  with Inactivir SD COMPLETE Viral Inactivation Buffer 2mL in 12mL  with swab in a pouch

Kit

KIT-VIB-004X-O

LSG Sample Collection Kit  with Inactivir SD COMPLETE Viral Inactivation Buffer 2mL in 7mL  with swab in a pouch

Kit

Inactivir® SDI includes an additional chaotrope along with the non-guanidine inactivation system presented in Inactivir SD LITE. This provides excellent stability for samples and allows the sample to be used directly in silica-based purification systems. 

This product is also available in bulk format if required.

Product code     Description

KIT-VIB-005G-N

LSG Sample Collection Kit  with Inactivir SDI STANDARD Viral Inactivation Buffer 1mL in 12mL  with swab in a pouch

Kit

KIT-VIB-005G-O

LSG Sample Collection Kit  with Inactivir SDI STANDARD Viral Inactivation Buffer 1mL in 7mL  with swab in a pouch

Kit

KIT-VIB-005U-N

LSG Sample Collection Kit  with Inactivir SDI STANDARD Viral Inactivation Buffer 3mL in 12mL  with swab in a pouch

Kit

KIT-VIB-005U-O

LSG Sample Collection Kit  with Inactivir SDI STANDARD Viral Inactivation Buffer 3mL in 7mL  with swab in a pouch

Kit

KIT-VIB-005X-N

LSG Sample Collection Kit  with Inactivir SDI STANDARD Viral Inactivation Buffer 2mL in 12mL  with swab in a pouch

Kit

KIT-VIB-005X-O

LSG Sample Collection Kit  with Inactivir SDI STANDARD Viral Inactivation Buffer 2mL in 7mL  with swab in a pouch

Kit

  • Successful inactivation of potentially infectious clinical samples is a critically important for all stages of sample handling, from the initial sample collection to processing and discarding of the residual material. This is an important consideration for all facilities conducting real-time qPCR assays.
  • The use of inactivation buffers for the collection and transportation of samples clearly has significant advantages for transportation through courier/postal systems, sample stability through the inactivation of the virus and the stabilisation of the RNA, and safer handling for operatives handling sample receipt and conducting the assays.
  • The use of Inactivir ® inactivation buffers provides the ability for safe handling of samples with no loss of sensitivity in the final assay. This is of particular importance in geographies with limited access to diagnostic laboratories and where stability and safety of samples collected is paramount.
  • In many cases, the use of Inactivir ® inactivation buffers reduces the cost and time involved in sample handling and assay by the removal of one or more of the processing steps in the assay protocol.

The LSG NAP buffer is a transport medium for preserving nucleic acids that can be stored reliably at room temperature after sampling.

NAP Transport medium can release objects from the head of the sample collection swab into the Nucleic acid preservation solution.   The sample can be safely transported through the use of a sample transport container, and the structure is convenient and centrifugal separation can be used using a self-standing mechanical tube.

This product contains antibiotic/antimycotic to protect against the grown of bacterial and fungi

Simply place the samples in the nucleic acid preservation medium. DNA will remain stable at room temperature for five months and RNA will remain stable for a week.

This product is also available in bulk format if required.

Product code     Description

KIT-LSG-1112G-N

LSG Sample Collection Kit  with Nucleic Acid Preservation (NAP) RNA/DNA Stabilisation Buffer 1mL in 12mL with swab in a pouch

Kit

KIT-LSG-1112G-O

LSG Sample Collection Kit with Nucleic Acid Preservation (NAP) RNA/DNA Stabilisation Buffer 1mL in 7mL with swab in a pouch

Kit

KIT-LSG-1112U-N

LSG Sample Collection Kit with Nucleic Acid Preservation (NAP) RNA/DNA Stabilisation Buffer 3mL in 12mL with swab in a pouch

Kit

KIT-LSG-1112U-O

LSG Sample Collection Kit with Nucleic Acid Preservation (NAP) RNA/DNA Stabilisation Buffer 3mL in 7mL with swab in a pouch

Kit

KIT-LSG-1112X-N

LSG Sample Collection Kit with Nucleic Acid Preservation (NAP) RNA/DNA Stabilisation Buffer 2mL in 12mL with swab in a pouch

Kit

KIT-LSG-1112X-O

LSG Sample Collection Kit with Nucleic Acid Preservation (NAP) RNA/DNA Stabilisation Buffer 2mL in 7mL with swab in a pouch

Kit

LSG can provide isotonic saline solution, 0.85% saline, both as a bulk solution or dispensed into tubes and presented in kit format with a swab for sample collection.  This product is intended to be inoculated with nasopharyngeal (NP) or oropharyngeal (OP) synthetic fibre swab specimens for further analysis by qRT-PCR assays.   This product is also available in bulk format if required.

Product code     Description

KIT-SAL-001G-O

LSG Sample Collection Kit  with 0.85% Saline Solution 1mL in 7mL with  swab in a pouch

Kit

KIT-SAL-001G-N

LSG Sample Collection Kit  with 0.85% Saline Solution 1mL in 12mL  with swab in a pouch

Kit

KIT-SAL-001X-N

LSG Sample Collection Kit  with 0.85% Saline Solution 2mL in 12mL  with swab in a pouch

Kit

KIT-SAL-001X-O

LSG Sample Collection Kit  with 0.85% Saline Solution 2mL in 7mL with  swab in a pouch

Kit

KIT-SAL-001U-N

LSG Sample Collection Kit  with 0.85% Saline Solution 3mL in 12mL  with swab in a pouch

Kit

KIT-SAL-001U-O

LSG Sample Collection Kit  with 0.85% Saline Solution 3mL in 7mL with  swab in a pouch

Kit