Wednesday, 10 January 2018 / Published in Human AB Serum
Human AB Serum is a vital cell culture reagent for some human cell types providing growth factors, vitamins, nutrients as well as trace elements and transport factors, ensuring faster growth rates than mixed blood group serum. Human AB is proven to grow many human cell lines at a faster rate and with a smaller percentage
Wednesday, 10 January 2018 / Published in Human AB Serum
US origin Human AB serum is collected from healthy volunteer male donors of the AB serotype at FDA-licensed facilities in the United States and collected in compliance with health requirements established by 21CFR 640, subpart G. Converted serum is routinely screened for the presence of animal contaminants. This is performed by DNA analysis. French origin
Wednesday, 10 January 2018 / Published in Human AB Serum
Each lot of human serum type AB of clot typically derives from 200-250 pre-screened donors. Smaller batches may be made available to a specific number of individual donors. Batches of Human AB serum from converted plasma may be constructed to have a smaller number of donors, potentially 10-15, and be reproducible.
Wednesday, 10 January 2018 / Published in Human AB Serum
Women who have been pregnant may develop antibodies against major histocompatibility class (MHC) antigens carried on the father’s cells and/or the foetus’ cells. Male donors present no such risks.
Wednesday, 10 January 2018 / Published in Human AB Serum
Human serum from type AB donors lacks antibodies against the A and B blood-type antigens and is therefore commonly used when there is the need to minimize immunoreactivity.
Wednesday, 10 January 2018 / Published in Human AB Serum
Off-the-clot serum is collected from blood that is allowed to coagulate naturally after collection. It has not been exposed to any anticoagulant. Plasma derived serum is produced by defibrinating pooled human blood collected in the presence of bovine thrombin. Plasma derived serum is generally more economical and popular than the off-the-clot product. Both Off-the-clot serum
Wednesday, 10 January 2018 / Published in Human AB Serum
Human AB serum supplied by LSG is suitable for research, diagnostic and further manufacturing purposes. Full details as to the collection and processing of human AB serum is available on request. Specific batches of serum may be subjected to additional viral testing, as required. LSG is very happy to discuss specific batch and collection requirements to
Wednesday, 10 January 2018 / Published in Human AB Serum
Human AB may be supplied in any bottle size e.g. 1 litre, 500 mL, 100 mL, 50 mL, 25 mL, as required, including bottles fitted with septums for the aseptic removal of serum.  Human AB serum may also be processed into single use bags with tubing sets to meet individual production requirements.
Wednesday, 10 January 2018 / Published in Human AB Serum
Recommended storage is -20°C or below. Protect serum from exposure to light. It is recommended to avoid freeze-thaw cycles as this can lead to a deterioration in serum qualities. Ideally, material should be thawed under controlled conditions and re-aliquoted into smaller volumes before re-freezing. It is not recommended to store or refreeze partially used serum
Wednesday, 10 January 2018 / Published in Human AB Serum
Manufacturing grade Human AB serum offers enhanced donor traceability and additional viral testing compared with standard grade.
Wednesday, 10 January 2018 / Published in Human AB Serum
Each batch of Human AB serum is tested to ensure freedom from bacteria, fungi, yeast, mycoplasma (M. pneumonia, M. hominis and M. salivarium). Batches are also tested for the ability to support growth of specific cell lines. In addition, each batch is also tested for standard parameters. These include endotoxin, pH, osmolality, protein content, albumin,
Wednesday, 10 January 2018 / Published in Human AB Serum
All donor units that form each batch are tested for HBsAG, anti-HCV, anti-HIV1 and 2, HIV-1, and Syphilis. Additional viral testing on both individual donors and pooled material is available to meet current regulatory standards for further manufacturing.
Wednesday, 10 January 2018 / Published in Human AB Serum
Each batch of Human AB serum is tested for the absence of bacteria, fungi, yeast, mycoplasma. Our Human AB serum is also sterile filtered to either 0.1 µm pore size-rated filters (US Origin) or 0.2 µm pore size-rated filters (French Origin). Results of microbe testing of each batch of serum is supplied in the COA.
Wednesday, 10 January 2018 / Published in Human AB Serum
Yes. Human AB serum is available as heat inactivated, gamma irradiated, dialysed and charcoal stripped material.
Wednesday, 10 January 2018 / Published in Human AB Serum
Human AB Serum may be heat inactivated by heating to 56°C for 30 minutes to inactivate various components in the serum such as complement factors.
Wednesday, 10 January 2018 / Published in Human AB Serum
At one time, heat inactivation was considered necessary because of concerns over the presence of contaminants in serum. However, the process of heat inactivation is known to degrade valuable biomolecules, such as growth factors, vitamins, and amino acids. Many protocols still call for serum to be subjected to heat treatment prior to use without consideration
Wednesday, 10 January 2018 / Published in Human AB Serum
Yes. We offer samples of Human AB Serum for testing prior to selection of a suitable batch. Typical sample size is 25 mL and reservations are held for a period of four weeks, pending evaluation.
Wednesday, 10 January 2018 / Published in Human AB Serum
Human AB Serum has a shelf life of 5 years from the date of manufacture, provided it is stored appropriately. We would recommend enquiring about the shelf life of each available batch if it is important to have a long shelf life following purchase.
Wednesday, 10 January 2018 / Published in Human AB Serum
Despite all testing, proper safety precautions for potentially infectious agents must be taken. All human blood products should be handled in accordance with currently acceptable bio-safety practices and guidelines for the prevention of blood borne viral infections.
Wednesday, 10 January 2018 / Published in Human AB Serum
US Origin The deferral time for any transfusion patient to stop donating blood products is 12 months. After this time, these donors can return to donating blood, serum or plasma. Transfusion grade collections don’t allow for transfused patients to donate. However, only some sites collect “transfusion grade” serum while others don’t. This means that blood