Allopregnanolone (AP) BioAssay ELISA Kit

£1,111.00 96 Tests

Allopregnanolone (AP) BioAssay ELISA Kit is a competitive inhibition immunoassay for in vitro quantitative measurement of AP in serum, plasma and other biological fluids.

SKU: US023253 Categories: , ,

Additional information

Detection Range

1.23-100ng/ml

Sensitivity

0.49ng/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components

*023253A: Microtiter Strips, 1×48 wells or 1×96 wells, Pre-coated, ready to use
*023253B: Standard, 2×1 vial
023253C: Standard Diluent, 1x10ml or 1x20ml
*023253D: Detection Reagent A (green), 1x60ul or 1x120ul
*023253E: Detection Reagent B (red), 1x60ul or 1x120ul
023253F: Assay Diluent A, 1x6ml or 1x12ml
023253G: Assay Diluent B, 1x6ml or 1x12ml
023253H: TMB Substrate, 1×4.5ml or 1x9ml
*023253J: Positive Control, 3.34ng/ml, 1x1vial
023253K: Stop Solution, 1x3ml or 1x6ml
023253L: Wash Buffer, 30X, 1x10ml or 1x20ml

Precaution

The Stop Solution (023253K) included for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *023253A, *023253B, *023253D, *023253E and *023253J at -20?C. After reconstitution of *023253J, store at 4?C and use within 5 days. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Notes on 023253J

Positive Control

Intended Use

The positive control is from a human serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.

Characteristics

The positive control vial contains human AP in human serum (lyophilized powder). Note: The human based material used in this product is screened for HIV, Hepatitis A, B, C, HTLV and Syphilis.

Expected Values

9.97ng/ml

Usage

Dissolve with 150ul standard diluent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 50ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20?C. After reconstitution, store Positive Control vial at 4?C and use within 5 days.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37?C.
3. Aspirate and wash 3 times.
4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
5. Aspirate and wash 5 times.
6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37?C.
7. Add 50ul Stop Solution. Read at 450nm immediately.

Shelf Life

6 Months