Allopregnanolone (AP) BioAssay ELISA Kit
£1,111.00 96 Tests
Allopregnanolone (AP) BioAssay ELISA Kit is a competitive inhibition immunoassay for in vitro quantitative measurement of AP in serum, plasma and other biological fluids.
Additional information
Detection Range | 1.23-100ng/ml |
---|---|
Sensitivity | 0.49ng/ml |
Precision | Intra-Assay CV: Inter-Assay CV: |
Kit Components | *023253A: Microtiter Strips, 1×48 wells or 1×96 wells, Pre-coated, ready to use |
Precaution | The Stop Solution (023253K) included for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. |
Storage and Stability | Store *023253A, *023253B, *023253D, *023253E and *023253J at -20?C. After reconstitution of *023253J, store at 4?C and use within 5 days. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. |
Notes on 023253J | Positive Control |
Intended Use | The positive control is from a human serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly. |
Characteristics | The positive control vial contains human AP in human serum (lyophilized powder). Note: The human based material used in this product is screened for HIV, Hepatitis A, B, C, HTLV and Syphilis. |
Expected Values | 9.97ng/ml |
Usage | Dissolve with 150ul standard diluent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 50ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20?C. After reconstitution, store Positive Control vial at 4?C and use within 5 days. |
Assay Procedure Summary | 1. Prepare all reagents, samples and standards. |
Shelf Life | 6 Months |