Mca-PLGL-Dap(Dnp)-AR-NH2

£125.00 1mg

FRET substrate for matrix metalloproteinases.

Mca-PLGL-Dap(Dnp)-AR-NH2 is a sensitive synthetic peptide FRET substrate for the determination of matrix metalloproteinase (MMP) activity. Protease cleavage at the -Gly-Leu- bond releases the highly fluorescent 7-methoxycoumarin (Mca) group from the influence of the 2,4-dinitrophenyl (Dnp) internal quencher, resulting in a 190 fold increase in fluorescence intensity. Mca-PLGL-Dap(Dnp)-AR-NH2 is suitable for use in continuous assays at enzyme concentrations comparable to those used with macromolecular substrates and also for the assessment of total matrix metalloproteinase in crude preparations.

SKU: PS-010 Categories: ,

Additional information

Three Letter Sequence

Mca-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2

Molecular Weight

1093.2

Molecular Formula

C49H68N14O15

Sequence

Mca-PLGLX(Dnp)AR-NH2

Solubility

Soluble in DMSO

Appearance

Freeze dried solid

Storage

Store desiccated, frozen and in the dark

Purity

>95% by HPLC

Modifications

Mca (7-methoxycoumarin) on N terminal, C terminal is amidated, X is diaminopropionic acid substituted with 2,4-dinitrophenyl (Dnp)

Searchable Words

Mca-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2, 140430-53-1, Mca-PLGL-Dap(Dnp)-AR-NH2, FRET substrate, matrix metalloproteinase, MMP, PS-010, PS010