Please note: this product can only be supplied to registered research and development facilities, and may be used for in vitro research use only, not for administration to humans or animals.

FRET substrate for matrix metalloproteinases.

Mca-KPLGL-Dap(Dnp)-AR-NH2 is a broad spectrum fluorogenic FRET substrate suitable for matrix metalloproteinases including intestinal collagenase (MMP-1), neutrophil collagenase (MMP-8) collagenase 3 (MMP-13), gelatinase A (MMP-2), gelatinase B (MMP-9), matrilysin (MMP-7) and MMP-17. Mca-KPLGL-Dap(Dnp)-AR-NH2 is cleaved very poorly by neutrophil elastase, and not at all by trypsin. Mca-KPLGL-Dap(Dnp)-AR-NH2 was obtained by the addition of an N-terminal lysine to Mca-PLGL-Dap(Dnp)-AR-NH (PS-040) which improves substrate properties. It contains the highly fluorescent 7-methoxycoumarin (Mca) group that is efficiently quenched by resonance energy transfer to the 2,4-dinitrophenyl (Dnp) group. Mca-KPLGL-Dap(Dnp)-AR-NH2 is fully water soluble and allows measurement of metalloproteinase activity in tissue culture conditions such as on the surface of cells.