Atto550-LPETGG

£125.00 0.1mg

Sortase reagent.

Atto550-LPETGG is the sortase sequence, LPETGG, coupled to the fluorescent label Atto550. Atto550-LPETGG can be used for site-specific labelling of target proteins using sortase-catalyzed transpeptidation. In this process, sortase A cleaves between the thr and gly residues, forming a thioester intermediate with the peptide probe. A protein N-terminally engineered with a series of glycine residues resolves this intermediate, regenerating the active site cysteine of sortase and conjugating the probe to the N-terminus of the protein. Atto550-LPETGG contains the Atto550 fluorescent label which is efficiently excited in the range 540 – 565 nm, with strong absorption, high fluorescence quantum yield and high photo-stability.

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SKU: FP-040 Categories: ,

Additional information

Three Letter Sequence

Atto550-Leu-Pro-Glu-Thr-Gly-Gly-OH

Molecular Weight

1148.6

Molecular Formula

C60H76N9O14

Sequence

Atto550-LPETGG

Solubility

Soluble in water

Appearance

Freeze dried solid

Storage

Store dry, frozen and in the dark

Purity

>95% by HPLC

Modifications

Atto550 at N terminal

Searchable Words

Atto550-LPETGG, FP-040