Ac-DEVD-AMC
£49.00 1mg
Please note: this product can only be supplied to registered research and development facilities, and may be used for in vitro research use only, not for administration to humans or animals.
Fluorogenic substrate for caspase-3.
Ac-DEVD-AMC is a fluorogenic substrate for caspase-3 with a Km value of 9.7 1.0 μM. The sequence of Ac-DEVD-AMC is based on the PARP cleavage site Asp216 for caspase-3 and it is also a substrate for caspases -1, -4, -7 and -8. Ac-DEVD-AMC can be used to monitor intracellular caspase activity with cleaved AMC moieties, reflecting caspase activity, being detected by fluorescence.
Please contact us for availability.
Additional information
| Three Letter Sequence | Ac-Asp-Glu-Val-Asp-AMC |
|---|---|
| Molecular Weight | 675.7 |
| Molecular Formula | C30H37N5O13 |
| Sequence | DEVD |
| Solubility | Soluble in DMSO; dilute with buffer for use |
| Appearance | Lyophilised solid |
| Storage | We recommend storage desiccated, frozen and in the dark |
| Purity | ‰¥97% (HPLC) |
| Modifications | Acetyl at N terminal, 7-amino-4-methylcoumarin (AMC) at C terminal |
| Searchable Words | DEVD, substrate, caspase-3 , 169332-61-0, Ac-DEVD-AMC, PI-260, PI260 |