Please note: this product can only be supplied to registered research and development facilities, and may be used for in vitro research use only, not for administration to humans or animals.

Sortase reagent.

Atto488-LPETGG is the sortase recognition sequence, LPETGG, coupled to the fluorescent label Atto488. Atto488-LPETGG can be used for site-specific labeling of target proteins using sortase-catalyzed transpeptidation. In this process, sortase A cleaves between the thr and gly residues, forming a thioester intermediate with the peptide probe. A protein N-terminally engineered with a series of glycine residues resolves this intermediate, regenerating the active site cysteine of sortase and conjugating the probe to the N-terminus of the protein. Atto488-LPETGG contains the Atto488 fluorescent label which is efficiently excited in the range 480 – 515 nm, with strong absorption, high fluorescence quantum yield and high photo-stability.

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