Vitronectin (VTN) BioAssay ELISA Kit (Human)

£990.00 96 Tests

Vitronectin (VTN) BioAssay ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of VTN in human serum, plasma and other biological fluids.

SKU: US028904 Categories: , ,

Additional information

Detection Range

7.8-500pg/ml

Sensitivity

3.2pg/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components

*028904A: Microtiter Strips, 1×96 wells, Pre-coated, ready to use
*028904B: Standard, 2×1 vial
028904C: Standard Diluent, 1x20ml *028904D: Detection Reagent A (green), 1x120ul *028904E: Detection Reagent B (red), 1x120ul
028904F: Assay Diluent A, 1x12ml
028904G: Assay Diluent B, 1x12ml
028904H: TMB Substrate, 1x9ml 0
28904K: Stop Solution, 1x6ml
028904L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (028904K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *028904A, *028904B, *028904D and *028904E at -20?C. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to eac well. Incubate 2 hours at 37?C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour a 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reag B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul Substrate Solution. Incubat 15-25 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen

Human

Specificity

Human

Shelf Life

6 Months