Trypsin (TRY) BioAssay ELISA Kit (Porcine)

£1,068.00 96 Tests

Trypsin (TRY) BioAssay ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TRY in porcine serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

SKU: US028641 Categories: , ,

Additional information

Detection Range

0.156-10ng/ml

Sensitivity

0.065ng/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

KIt Components

*028641A: Microtiter Strips, 1×96 wells, Pre-coated, ready to use
*028641B: Standard, 2x1vial?
028641C: Standard Diluent, 1x20ml
*028641D: Detection Reagent A (green), 1x120ul?
*028641E: Detection Reagent B (red), 1x120ul?
028641F: Assay Diluent A, 1x12ml
028641G: Assay Diluent B, 1x12ml
028641H: TMB Substrate, 1x9ml
*028641J: Positive Control, 16.2ng/ml, 1x1vial (Lyophilized)
028641K: Stop Solution, 1x6ml
028641L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (028641K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *028641A, *028641B, *028641D, *028641E and *028641J at -20°C. Store all the other components at 4°C. After reconstitution of *028641J, store at 4°C and use within 5 days. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Notes on 028641J

Positive Control

Intended Use

The control vial is from a porcine serum sample known to contain TRY. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.

Characteristics

The control vial contains porcine TRY in porcine serum (lyophilized powder). Note: The porcine source material used in this product is from healthy porcine of SPF standards (specific pathogen-free animals).

Expected Values

16.2ng/ml

Usage

Dissolve with 150ul standard diluent and let it stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens.
Note: Upon receipt store Positive Control vial at -20?C. After reconstitution, store Positive Control vial at 4?C and use within 5 days.

Assay Procedure Summary

1. Prepare all reagents, samples and standards;
2. Add 100ul standard or sample to each well. Incubate 1 hour at 37?C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Shipping Temperature

On Dry Ice

Shelf Life

6 Months