Triiodothyronine, Free, Human, BioAssay ELISA Kit (fT3, Free T3)

£927.22 96 Tests

Triiodothyronine, Free, Human, BioAssay ELISA Kit (fT3, Free T3) is based on competitive binding of human free thyroxine from serum samples and enzyme-labeled T3 to T3-specific antibodies immobilized on microtiter well plates. After a washing step, chromogenic substrate is added and color developed. The enzymatic reaction (blue color) is inversely proportional to the amount of T3 present in the sample. The reaction is terminated by adding stopping solution (converts blue to yellow). Absorbance is then measured on a microtiter well ELISA reader at 450nm. and the concentration of free T3 in samples and control is read off the standard curve.

SKU: UST8425-06 Categories: ,

Additional information

Sensitivity

0.3pg/ml

Range

2-40pg/ml

Storage and Stability

Store all components except standards and control at 4°C. Aliquot Standards and Controls storing at -20°C. Stable for a 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shelf Life

6 Months

Source Antigen

Human

Kit Components

T8425-06A1: Microstrip Plate, 1x96wells
T8425-06A2: Assay Buffer, 1x15ml
T8425-06A3: Sample Diluent/Standard 0pg/ml 1x10ml
T8425-06A4: Standard 2pg/ml 1x500ul
T8425-06A5: Standard 4pg/ml 1x500ul
T8425-06A6: Standard 8pg/ml 1x500ul
T8425-06A7: Standard 16pg/ml 1x500ul
T8425-06A8: Standard 40pg/ml 1x500ul
T8425-06A9: Low Control (3.5-5.5pg/ml), 1x500ul
T8425-06A10: High Control (6.3-10.5pg/m). 1x500ul
T8425-06A11: Ab (HRP), 50X, 1x300ul
T8425-06A12: Wash Buffer, 10X, 1x50ml
T8425-06A13: TMB Solution, 1x16ml
T8425-06A14: Stop Solution (1M sulfuric acid), 1x6ml