Trefoil Factor 3, Intestinal (TFF3) BioAssay ELISA Kit (Mouse)

£1,007.00 96 Tests

Trefoil Factor 3, Intestinal (TFF3) BioAssay ELISA Kit (Mouse) is a sandwich enzyme immunoassay technique for the in vitro quantitative measurement of mouse TFF3 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

SKU: US028606 Categories: , ,

Additional information

Detection Range

0.312-20ng/ml

Sensitivity

0.125ng/ml

Precision

Intra-Assay:
CV<10%

Inter-Assay:
CV<12%

Kit Components

*028606A: Microtiter Strip, 1×96 wells, Pre-coated, ready to use
*028606B: Standard, 2x1vial?
028606C: Standard Diluent, 1x20ml
*028606D: Detection Reagent A, 1x120ul?
*028606E: Detection Reagent B, 1x120ul?
028606F: Assay Diluent A, 1x12ml
028606G: Assay Diluent B, 1x12ml
028606H: TMB Substrate, 1x9ml
028606K: Stop Solution, 1x6ml
028606L: Wash Buffer, 30X, 1x20ml

Storage and Stability

Store *028606A, *028606B, *028606D and *028606E at -20?C. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Summary

1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37?C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen

Mouse

Shelf Life

6 Months