Transferrin Receptor, Soluble, Human, BioAssay ELISA Kit (TfR1, TFRC, CD71, TR, Tfr, TfnrR, sTfR)

£989.66 1 Kit

The transferrin receptor (TfR) is the gateway for transferrin-bound-iron entering all body cells. TfR is abundant on the surface of many newly formed cells, but the erythroid marrow cells account for 70 to 80 % of the total body TfR content. The soluble (or serum) transferrin receptor (sTfR) is a circulating truncated form of the membrane receptor protein; it is an 85kD glycoprotein forming in serum a 320kD complex with diferric transferrin. The serum sTfR concentration reflects the total body mass of cellular transferrin receptor. Anaemias associated with enhanced erythropoiesis and iron deficiency result in an elevation in the sTfR values. Elevation of the soluble transferrin receptor may be also caused by haemolytic anaemia, polycythaemia and thalassemia while aplastic anaemia and chronic renal failure may result in its decrease. The most important clinical use of the sTfR determination is in the differential diagnosis between iron deficiency anaemia and the anaemia of chronic disease.

The Human sTfR BioAssay ELISA is a sandwich enzyme immunoassay for the quantitative measurement of soluble human transferrin receptor in serum and plasma (EDTA, heparin, citrate).

SKU: UST8199-38 Categories: ,

Description

Test Principle:
Standards, quality controls and samples of sera are incubated in microtitration wells coated with monoclonal anti-human sTfR antibody. After a thorough wash, monoclonal anti-human sTfR antibody labeled with horseradish peroxidase (HRP) is added to the wells and incubated with the immobilized antibody?sTfR complex. Following another washing step, the remaining HRP-conjugated antibody is allowed to react with the substrate H2O2 and tetramethylbenzidine. The reaction is stopped by addition of acidic solution, and absorbance of the resulting yellow colour product is measured spectrophotometrically at 450nm. The absorbance is proportional to the concentration of sTfR. A standard curve is constructed by plotting absorbance values versus recombinant sTfR concentrations of standards, and concentrations of unknown samples are determined using this standard curve.

Additional information

Detection Range

0.05-2ug/ml

Sensitivity

2ng/ml

Specificity

Human soluble transferrin receptor

Storage and Stability

Store kit at 4°C. Unopened kit components are stable for 6 months after receipt. Opened reagents are stable for 3 months when stored at 4°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Shipping Temperature

On Dry Ice

Kit Components

T8199-38A: Microtiter Strips, 1x 96 wells, coated with anti-Hu sTfR Mab
T8199-38B: Mab (HRP), 1x13ml
T8199-38C: Standard, 2ug/ml, 1x100ul
T8199-38D: Standard, 1ug/ml, 1x100ul
T8199-38E: Standard, 0.5ug/ml, 1x.100ul
T8199-38F: Standard, 0.2ug/ml, 1x100ul
T8199-38G: Standard, 0.1ug/ml, 1x100ul
T8199-38H: Standard, 0.05ug/ml, 1x100ul
T8199-38J: Quality Control, High, 50X, 1x50ul
T8199-38K: Quality Control, Low, 50X, 1x50ul
T8199-38L: Dilution Buffer, 2x13ml
T8199-38M: Wash Solution, 10X, 1x100ml
T8199-38N: Substrate Solution (TMB), 1x13ml
T8199-38P: Stop Solution, 1x13ml