Thrombospondin 1 (THBS1) BioAssay ELISA Kit (Rat)

£1,007.00 96 Tests

Thrombospondin 1 (THBS1) BioAssay ELISA Kit (Rat) is a sandwich enzyme immunoassay for in vitro quantitative measurement of THBS1 in rat plasma.

Additional information

Detection Range

15.6-1000pg/ml

Sensitivity

5.3pg/ml

Precision

Intra-Assay:
CV < 10%

Inter-Assay:
CV < 12%

Kit Components

*028361A: Microtiter Plate, 1×96 wells, Pre-coated, ready to use
*028361B: Standard, 2×1 vial?
028361C: Standard Diluent, 1x20ml
*028361D: Detection Reagent A (100X), 1x120ul?
*028361E: Detection Reagent B (100X), 1x120ul?
028361F: Assay Diluent A (2X), 1x6ml
028361G: Assay Diluent B (2X), 1x6ml
028361H: TMB Substrate, 1x9ml
*028361J: Positive Control, 61.98pg/ml, 1×1 vial
028361K: Stop Solution, 1x6ml
028361L: Wash Buffer (30X), 1x20ml

Precaution

The Stop Solution (028361K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *028361A, *028361B, *028361D, *028361E and *028361J at -20°C. After reconstitution of *028361J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards;
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37?C.
3. Add 100ul working Detection Reagent A immediately. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul working Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Shipping Temperature

On Dry Ice

Source Antigen

Rat

Specificity

Rat

Shelf Life

6 Months