Thrombospondin 1 (THBS1) BioAssay ELISA Kit (Mouse)

£976.00 96 Tests

Thrombospondin 1 (THBS1) BioAssay ELISA Kit (Mouse) is a sandwich enzyme immunoassay for in vitro quantitative measurement of THBS1 in mouse plasma.

Additional information

Detection Range

1.25-80ng/ml

Sensitivity

0.44ng/ml

Precision

Intra-Assay CV:
< 10%

Inter-Assay CV:
< 12%

Kit Components

*028362A: Microtiter Strips, 1×96 wells, Pre-coated, ready to use
*028362B: Standard, 2×1 vial
028362C: Standard Diluent, 1x20ml
*028362D: Detection Reagent A, 1x120ul
*028362E: Detection Reagent B, 1x120ul
028362F: Assay Diluent A, 1x12ml
028362G: Assay Diluent B, 1x12ml
028362H: TMB Substrate, 1x9ml
028362K: Stop Solution, 1x6ml
028362L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (028362K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *028362A, *028362B, *028362D and *028362E at -20?C. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards;
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37?C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Shipping Temperature

On Dry Ice

Source Antigen

Mouse

Specificity

Mouse

Shelf Life

6 Months