Terminal Complement Complex C5b-9 (C5b-9) BioAssay ELISA Kit (Rat)

£1,071.00 96 Tests

The Rat Terminal Complement Complex C5b-9 (C5b-9) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of C5b-9 in rat serum, plasma and other biological fluids.

SKU: US028320 Categories: , ,

Additional information

Detection Range

31.2-2000pg/ml

Sensitivity

13.5pg/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components

*028320A: Microtiter Plate, 1×96 wells, Pre-coated; ready to use
*028320B: Standard, 2x1vial
028320C: Standard Diluent, 1x20ml
*028320D: Detection Reagent A (green), 1x120ul
*028320E: Detection Reagent B (red), 1x120ul
028320F: Assay Diluent A, 1x12ml
028320G: Assay Diluent B, 1x12ml
028320H: TMB Substrate, 1x9ml
028320K: Stop Solution, 1x6ml
028320L: Wash Buffer, 30x, 1x20ml

Precaution

The Stop Solution (028320K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *028320A, *028320B, *028320D and *028320E at -20?C. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37?C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen

Rat

Shelf Life

6 Months