Surfactant Associated Protein A (SPA) BioAssay ELISA Kit (Human)

£960.00 96 Tests

Surfactant Associated Protein A (SPA) BioAssay ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of SPA in human serum, plasma, tissue homogenates, cell lysates, lung lavage fluid, cell culture supernates and other biological fluids.

SKU: US028237 Categories: , ,

Additional information

Detection Range

46.88-3000pg/ml

Sensitivity

17.43pg/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components

*028237A: Microtiter Strips, 1×96 wells, Pre-coated, ready to use
*028237B: Standard, 2×1 vial
028237C: Standard Diluent, 1x20ml
*028237D: Detection Reagent A (green), 1x120ul
*028237E: Detection Reagent B (red), 1x120ul
028237F: Assay Diluent A, 1x12ml
028237G: Assay Diluent B, 1x12ml
028237H: TMB Substrate, 1x9ml
*028237J: Positive Control, 220.7pg/ml, 1x1vial (Lyophilized)
028237K: Stop Solution, 1x6ml
028237L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (028237K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *028237A, *028237B, *028237D, *028237E and *028237J at -20?C. After reconstitution of *028237J, store at 4?C and use within 5 days. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 1 hour at 37?C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul TMB Substrate. Incubate 10-20 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen

Human

Specificity

Human

Shelf Life

6 Months