Secretory Leukocyte Peptidase Inhibitor (SLPI) BioAssay ELISA Kit (Human)

£990.00 96 Tests

Secretory Leukocyte Peptidase Inhibitor (SLPI) BioAssay ELISA Kit (Human) is a sandwich enzyme immunoassay for the in vitro quantitative measurement of SLPI in human serum, plasma and other biological fluids.

SKU: US027994 Categories: , ,

Additional information

Detection Range

62.5-4000pg/ml

Sensitivity

29.2pg/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components

*027994A: Microtiter Plate, 1×96 wells, Pre-coated; ready to use
*027994B: Standard, 2x1vial
027994C: Standard Diluent, 1x20ml
*027994D: Detection Reagent A (green), 1x120ul
*027994E: Detection Reagent B (red), 1x120ul
027994F: Assay Diluent A, 1x12ml
027994G: Assay Diluent B, 1x12ml
027994H: TMB Substrate, 1x9ml
027994K: Stop Solution, 1x6ml
027994L: Wash Buffer, 30X, 1x20ml

Storage and Stability

Store *027994A, *027994B, *027994D and *027994E at -20?C. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure

1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37?C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen

Human

Shelf Life

6 Months