Secretin (SCT) BioAssay ELISA Kit (Human)

£990.00 96 Tests

Secretin (SCT) BioAssay ELISA Kit (Human) is a competitive inhibition immunoassay for in vitro quantitative measurement of SCT in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

SKU: US027983 Categories: , ,

Additional information

Detection Range

18.52-1500pg/ml

Sensitivity

7.36pg/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components

*027983A: Microtiter Plate, 96 wells, Pre-coated, ready to use
*027983B: Standard, 2×1 vial
027983C: Standard Diluent, 1x20ml
*027983D: Detection Reagent A (green), 1x120ul
*027983E: Detection Reagent B (red), 1x120ul
027983F: Assay Diluent A, 1x12ml
027983G: Assay Diluent B, 1x12ml
027983H: TMB Substrate, 1x9ml
027983K: Stop Solution, 1x6ml
027983L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (027983K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *027983A, *027983B, *027983D and *027983E at -20?C. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards;
2. Add 50ul standard or sample to each well.
3. Add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen

Human

Shelf Life

6 Months