Rheumatoid Factor (RF), Mouse, BioAssay ELISA Kit

£1,159.80 1 Kit

Rheumatoid Factors (RFs) were described as Immunoglobulins (IgGs) in rheumatoid arthritis as detected by agglutination of rabbit IgG coated erythrocytes. It was later demonstrated that RFs were IgM molecules reactive with the Fc-portion of the IgG from various species. In addition to the IgM RF, subsequent studies have identified RFs of other IgG subtypes (IgG, IgA, and IgE) in serum of patients with rheumatoid arthritis, and certain other chronic diseases. Most commercial assays measure predominantly IgM RFs. It has been observed that measurement of IgG RF may be a better indicator of rheumatoid arthritis than IgM RF. IgA RF and IgE RF have been implicated in articular disease process in rheumatoid arthritis and rheumatoid vasculitis, respectively. RFs, produced by cells within the joint space, are found in joint fluids as well. RF titer, particularly IgG RF correlate with the intensity of synovitis.

RFs can be found in serum samples from a variety of autoimmune diseases including rheumatoid arthritis, Systemic lupus erythematous, Sjogren’s syndrome, mixed connective tissue disease, mixed essential cryoglobulinemia, scleroderma, subacute bacterial endocarditis, leprosy, pulmonary fibrosis, and pulmonary silicosis. RFs may also be found in patients with syphilis, viral infections, chronic liver diseases, sarcoidosis, leprosy, neoplasms, and a variety of other chronic conditions.

Rheumatoid Factor (RF), BioAssay ELISA Kit is a sandwich ELISA for detecting and qualifying RF (IgG, IgM and/or IgA) activity in mouse serum and plasma.

SKU: USR1997-05 Categories: , ,

Additional information

Kit Components

R1997-05A: Microwell Strips, 1×96 wells
R1997-05B: RF Sensitivity Control, 1x1ml
R1997-05C: Positive Control, 1x1ml
R1997-05D: Sample Diluent, 20X, 1x10ml
R1997-05E: IgG (HRP), 100X GtxMo, 1x150ul
R1997-05F: Wash Buffer, 100X, 1x10ml
R1997-05G: TMB Substrate, 1x12ml
R1997-05H: Stop Solution. 1x12ml

Storage and Stability

Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Shipping Temperature

On Dry Ice

Shelf Life

6 Months