Rheumatoid Factor IgM, Human, BioAssay ELISA Kit

£1,081.76 96 Tests

Intended Use:
For quantitative determination of Rheumatoid Factor IgM in human serum.

Test Principle:
Rheumatoid Factor IgM (RF IgM) ELISA kit is based on binding of RF IgM from serum samples to human gamma globulin immobilized on microtiter wells. After a washing step, anti-human IgM-HRP conjugate is added. After another washing step, to remove all the unbound enzyme conjugate, chromogenic substrate is added and color developed. The enzymatic reaction (color) is directly proportional to the amount of RF IgM present in the sample. Adding stopping solution terminates the reaction. Absorbance is then measured on a microtiter well ELISA reader at 450nm and the concentration of RF IgM in samples is calculated on the basis of the absorbance of the negative, positive, and, calibrator controls.

SKU: USR1997-06 Categories: , ,

Additional information

Kit Components

R1997-06A: Microwell plate, 1×96 wells
R1997-06B: Negative Control (<25IU/ml)(100X), 1x300ul
R1997-06C: Positive Control (40-90IU/ml) (100X), 1x300ul
R1997-06D: Calibrator (100IU/ml) (100X), 1x300ul
R1997-06E: Sample Diluent, 1x50ml
R1997-06F: (HRP) Conjugate, 1x11ml
R1997-06G: Wash Buffer (100X), 1x50ml
R1997-06H: TMB Substrate, 1x11ml
R1997-06J: Stop solution, 1x10ml

Storage and Stability

Store all components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Shipping Temperature

On Dry Ice

Source Antigen

Human

Shelf Life

6 Months