Retinol Binding Protein 4 BioAssay ELISA Kit (Human) (RBP4)

£1,406.44 1 Kit

Retinol Binding Protein 4 BioAssay ELISA Kit (Human) (RBP4) is for the quantitative determination of human Retinol-Binding Protein 4 (RBP4) concentrations in cell culture supernates, serum, plasma, urine, and saliva.

Principle of the Assay:
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for RBP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RBP4 present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked monoclonal antibody specific for RBP4 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RBP4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

SKU: USR1701-36 Categories: , ,

Additional information

Assay Range

1.56-100ng/mL (Serum, plasma, Saliva, Cell Culture Supernates and Urine)

Specificity

Natural and recombinant human RBP4

Kit Components

R1701-36A1: Microtiter Strips 1×96 wells
R1701-36A2: Mab (HRP) Conjugate, 1x21ml
R1701-36A3: Standard (lyophilized), 1x200ng
R1701-36A4: Assay Diluent, 2x11ml
R1701-36A5: Calibrator Diluent, 1x21ml
R1701-36A6: Wash Buffer Concentrate (25X), 1x 21ml
R1701-36A7:Color Reagent A, (H2O2) 1x12ml
R1701-36A8: Color Reagent B (TMB), 1x12ml
R1701-36A9: Stop Solution (2N H2SO4) 1x6ml

Storage and Stability

Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Temperature

On Dry Ice

Shelf Life

6 Months