Protein C BioAssay ELISA Kit (Antibodies only)

£1,034.93 1 Kit

Protein C (PC) is a vitamin K-dependent glycoprotein produced in the liver. The concentration of PC in plasma is ~4ug/ml (~60 nM). A deficiency of Protein C (quantitative or qualitative) is a risk factor for vascular thrombosis. Protein C is expressed as a two-chain molecule with a molecular weight of 62 kD. The light chain (21kD of PC consists of two EGF-like domains and an amino-terminal domain containing one hydroxyaspartic acid and 11 ?-carboxyglutamic acid (gla) residues. These residues allow PC to bind to membranes that contain acidic phospholipids in a calcium dependent manner. The heavy chain of PC (41 kD) consists of the catalytic domain and an activation peptide. Activation of Protein C results from cleavage at residue Arg12 in the heavy chain by a complex of thrombin and a cell surface cofactor thrombomodulin.

The activation of PC is associated with the release of a small activation peptide (2-3 kD, called Protein C peptide, or PCP) from the N-terminal of the heavy chain. Activated Protein C (APC) is a serine protease with anticoagulant activity. APC, in complex with a phospholipid membrane, calcium and the Protein S cofactor, exhibits anticoagulant activity through the proteolytic inactivation of coagulation cofactors Va and VIIIa. The primary inhibitor of APC activity in plasma is Protein C Inhibitor (PCI, also called Plasminogen Activator Inhibitor-3, PAI-3) and to a lesser extent by ?1antitrypsin and ?2macroglobulin. The inhibitory activity of PCI is stimulated approximately 10 fold by heparin1-3.

SKU: USP9102-38 Categories: , ,

Additional information

Materials Supplied

Antibodies for 5×96 well plates

Kit Components

P9102-38A: PC Capture Antibody: 1x500ul Supplied as a liquid in 50% glycerol. Purified by immunoaffinity chromatography. PS IgG Pab ShxHu for coating ELISA plates.

P9108-07B: PC (HRP) Dection Antibody: 1x500ul Supplied as a liquid in 50% glycerol. Purified by immunoaffinity chromatography. PS (HRP) GtxHu for detection of captured PS.

Storage and Stability

For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Temperature

On Dry Ice

Shelf Life

6 Months