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Platelet Matrix: 3D Cell Culture System

Platelet Matrix is a lyophilized cell culture supplement derived from human Platelet Rich Plasma (PRP) which is subjected to a freeze-thaw process to induce growth factors release. The system provides a suitable 3D-matrix for cell culture that consists of the same components as the over-layered culture medium.

Replacing bovine serum with human Platelet Lysate (hPL) allows for expansion of stem cells without the risk of xenogeneic immune reactions or the potential for transmission of bovine pathogens. Biomaterials have an impact on proliferation and differentiation of cell preparations. Stem cells are routinely cultured on conventional tissue culture plastic (TCP). In these dishes, plastic adherent cell growth is restricted to two dimensions and it occurs preferentially at the rim of colonies due to contact inhibition. Furthermore, adherent cells need to be loosened from the substrate for passaging – usually by enzymatic treatment with peptidases such as trypsin.

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