Cell Culture Media
To ensure reproducible results in cell culture applications, media needs to be pure, contaminant-free, and consistent from lot to lot. Life Science Production provides a full range of high quality reagents and media products for mammalian, plant and insect cell culture applications.
Cell Culture Media products:
Balanced Salt Solutions (BSS)
Balanced salts are a combination of inorganic salts and normally a carbohydrate. They act as buffer to protect cells from sharp fluctuations in pH while maintaining the proper osmotic pressure across the cell membrane. In a complete medium, balanced salts are essential to the growth and maintenance of cells in culture. Alone balanced salts are generally used as a cell rinsing fluid and for short-term maintenance while cells are manipulated outside a complete growth environment.
Cell Specific Media
Cells present in the tissues have different physiological backgrounds. In addition, each cell type has specific metabolic pathways resulting in specific requirements for the supply of nutrients or supplementation with growth factors. LSP offers a range of special media, which guarantees optimal culture conditions even for sensitive cells.
Mammalian and insect cells are cryopreserved for a variety of reason. These include long-term storage, to minimise genetic changes in cell lines that are continuous thereby avoiding transformation of a cell line and to avoid loss of cells by contamination. It is very important that the correct type of cryopreservative is used to ensure successful cryopreservation and recovery.
Dulbecco’s Modified Eagle’s Medium (DMEM)
Dulbecco’s Modified Eagle’s Medium, High Glucose (DME/HIGH) contains a four-fold increase in the concentration of amino acids and vitamins found in Eagle’s Basal Medium (BME). DME media were originally developed to be used with a serum supplement in a 10% C02 atmosphere for the cultivation of non-transformed mouse and chicken cells. DMEM and its modifications are widely used to support the growth of a broad spectrum of mammalian cells.
Ham’s Nutrient Mixture
Hams nutrient mixture was originally designed for the serum-free growth of Chinese Hamster Ovary cells (CHO), HeLa cells and mouse cells. Supplemented with whole or dialysed serum or in combination with hormones and growth factors, Ham’s is widely used for the growth of a broad spectrum of mammalian and hybridoma cells. For optimum buffering, use in a 5% CO2 atmosphere.
Insect cells such as Sf21 and Sf9 are popular research tools in the study of viruses as well as for recombinant protein expression. LSP offers cell culture media specifically formulated to provide robust cell growth and protein production.
Iscove’s Modified Dulbecco’s Medium (IMDM)
Iscove’s Modified Dulbecco’s Medium is a modification of DME using high glucose (4500 mg/l).It contains sodium pyruvate and additional amino acids, HEPES buffer, selenium and other components. IMDM was originally designed for the serum-free growth of primary hematopoietic cells when, properly supplemented. When used in conjunction with serum ,IMDM supports the growth of a broad spectrum of mammalian cells. IMDM is ideal for rapidly proliferating high density cell cultures in a 5% CO2 atmosphere.
Islet Media & Reagents
Islet cell isolation and processing for diabetes research requires a variety of specialized cell culture media, separation and gradient solutions, procaine-specific solutions and related isolation and storage/preservation solutions.
Minimum Essential Medium (MEM)
Minimum Essential Medium was developed by Harry Eagle as a modification of his BME medium. MEM is a non-complex medium well suited for a wide range of mammalian cells when used with a serum supplement. MEM with EBSS is designed for use in a 5% CO2 atmosphere; MEM with HBSS is designed for use in an air atmosphere in closed containers.
Originally designed for the growth of human leukemic cells in monolayer or suspension cultures using a serum supplement, it has since demonstrated universal use in the growth and support of a broad spectrum of mammalian and hybrid cells as well as in hybridoma fusion protocols.