Procollagen I C-Terminal Propeptide (PICP) BioAssay ELISA Kit (Rat)

£1,007.00 96 Tests

Procollagen I C-Terminal Propeptide (PICP) BioAssay ELISA Kit (Rat) is a sandwich enzyme immunoassay for in vitro quantitative measurement of PICP in rat serum, plasma and other biological fluids.

SKU: US027592 Categories: , ,

Additional information

Detection Range

1.25-80ng/ml

Sensitivity

0.47ng/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components

*027592A: Microtiter Strip, 1×96 wells
*027592B: Standard, 2x1vial
027592C: Standard Diluent, 1x20ml
*0027592D: Detection Reagent A (green), 1x120ul
*027592E: Detection Reagent B (red), 1x120ul
027592F: Assay Diluent A, 1x12ml
027592G: Assay Diluent B, 1x12ml
027592H: TMB Substrate, 1x9ml
027592K: Stop Solution, 1x6ml
027592L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (027592K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *027592A, *027592B, *027592D and *027592E at -20?C. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards;
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37?C.
3. Add 100ul prepared Detection Reagent A immediately. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
5. Aspirate and wash 5 times.
6. Add 90ul 027590H: TMB Substrate. Incubate 15-25 minutes at 37?C.
7. Add 50ul 027590K: Stop Solution. Read at 450nm immediately.

Source Antigen

Rat

Shelf Life

6 Months