Podocalyxin (PCX) BioAssay ELISA Kit (Human)

£960.00 96 Tests

Podocalyxin (PCX) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PCX in human serum, plasma, tissue homogenates, urine and other biological fluids.

SKU: US027538 Categories: , ,

Additional information

Detection Range

0.156-10ng/ml

Sensitivity

0.063ng/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components

*027538A: Microtiter Plate, 1×96 wells, Pre-coated; ready to use
*027538B: Standard, 2×1 vial
027538C: Standard Diluent, 1x20ml
*027538D: Detection Reagent A (green), 1x120ul
*027538E: Detection Reagent B (red), 1x120ul
027538F: Assay Diluent A, 1x12ml
027538G: Assay Diluent B, 1x12ml
027538H: TMB Substrate, 1x9ml
027538K: Stop Solution, 1x6ml
027538L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (027538K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *027538A, *027538B, *027538D and *027538E at -20?C. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37?C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen

Human

Shelf Life

6 Months