Peroxisome Proliferator Activated Receptor gamma (PPARg) BioAssay ELISA Kit (Human)

£960.00 96 Tests

Peroxisome Proliferator Activated Receptor gamma (PPARg) BioAssay ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of PPARg in human tissue homogenates, cell lysates and other biological fluids.

Additional information

Detection Range





Intra-Assay CV:

Inter-Assay CV:

Kit Components

*027382A: Microtiter Strips, 1×96 wells, Pre-coated, ready to use
*027382B: Standard, 2×1 vial
027382C: Standard Diluent, 1x20ml
*027382D: Detection Reagent A (green), 1x120ul
*027382E: Detection Reagent B (red), 1x120ul
027382F: Assay Diluent A, 1x12ml
027382G: Assay Diluent B, 1x12ml
027382H: TMB Substrate, 1x9ml
027382K: Stop Solution, 1x6ml
027382L: Wash Buffer, 30X, 1x20ml


The Stop Solution (027382K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *027382A, *027382B, *027382D and *027382E at -20?C. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37?C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen


Shelf Life

6 Months