Osteocalcin (OC) BioAssay ELISA Kit (Rat)

£1,007.00 96 Tests

Osteocalcin (OC) BioAssay ELISA Kit (Rat) is a sandwich enzyme immunoassay for in vitro quantitative measurement of OC in rat serum, plasma, tissue homogenates, cell lysates and other biological fluids.

SKU: US027243 Categories: , ,

Additional information

Detection Range

15.6-1000pg/ml

Sensitivity

6.3pg/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components

*027243A: Microtiter Strips, 1×96 wells, Pre-coated, ready to use
*027243B: Standard, 2×1 vial
027243C: Standard Diluent, 1x20ml
*027243D: Detection Reagent A (green), 1x120ul
*027243E: Detection Reagent B (red), 1x120ul
027243F: Assay Diluent A, 1x12ml
027243G: Assay Diluent B, 1x12ml
027243H: TMB Substrate, 1x9ml
*027243J: Positive Control, 162.8pg/ml, 1×1 vial (Lyophilized)
027243K: Stop Solution, 1x6ml
027243L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (027243K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *027243A, *027243B, *027243D, *027243E and *027243J at -20?C. After reconstitution of *027243J, store at 4?C and use within 5 days. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 1 hour at 37?C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Shelf Life

6 Months