Neprilysin (NEP) BioAssay ELISA Kit (Human)

£990.00 96 Tests

The Macrophage Inflammatory Protein 1 Alpha (NEP) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of NEP in human serum, plasma, cerebrospinal fluid and other biological fluids.

SKU: US027037 Categories: , ,

Additional information

Detection Range

31.2-2000pg/ml

Sensitivity

12.5pg/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components

*027037A: Microtiter Strips, 1×96 wells, Pre-coated, ready to use
*027037B: Standard, 2x1vial
027037C: Standard Diluent, 1x20ml
*027037D: Detection Reagent A (green), 1x120ul
*027037E: Detection Reagent B (red), 1x120ul
027037F: Assay Diluent A, 1x12ml
027037G: Assay Diluent B, 1x12ml
027037H: TMB Substrate, 1x9ml
*027037J: Positive Control, 386pg/ml, 1x1vial (Lyophilized)
027037K: Stop Solution, 1x6ml
027037L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (027037K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *027037A, *027037B, *027037D, *027037E and *027037J at -20?C. After reconstitution of *027037J, store at 4?C and use within 5 days. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Notes on 027037J

Positive Control

Intended Use

The control vials are from a human serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.

Characteristics

The control vials contain human NEP in human serum (lyophilized powder). Note: The human based serum used in this product are screened for HIV, Hepatitis A, B, C, HTLV and Syphilis.

Expected Values

386pg/ml

Usage

Dissolve with 150ul standard diluent and let stand for 10 minutes. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and samples. Note: Upon receipt store Positive Control vial at -20?C. After reconstitution, store Positive Control vial at 4?C and use within 5 days.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 1 hour at 37?C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul TMB Substrate. Incubate 10-20 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Shelf Life

6 Months