Neopterin, Human, BioAssay ELISA Kit

£1,236.29 96 Tests

Neopterin [D-erythro-neopterin] belongs to the group of pteridines. Neopterin as well as other pteridines are derived in vivo from guanosine-triphophate (GTP). Neopterin represents a precursor molecule of biopterin that is an essential cofactor in neurotransmitter synthesis, and it is also involved in variety of oxidation-reduction reactions in the body. The enzyme GTP-cyclohydrolase-I catalyzes this reaction in monocytes and macrophages. Neopterin is exclusively released by activated macrophages, and can be determined in serum, plasma and other body fluids. Neopterin biosynthesis is closely associated with activation of the cellular immune system. Higher levels of neopterin were found in patients with viral infections. Antigenic stimulation of human peripheral blood mononuclear cells leads to neopterin release into cell culture medium. Gamma Interferon treatment of macrophages in vitro also increases neopterin levels and generates highly reactive oxygen species (ROS). Neopterin is capable of enhancing ONOO- – as well as Cu(II)-mediated LDL oxidation, whereas 7,8-dihydroneopterin mainly protects LDL from oxidation.

Changes in neopterin concentrations in serum or urine can predict complications such as graft rejection in organ transplant recipients. Elevated neopterin levels are found in autoimmune disorders such as rheumatoid arthritis and systemic lupus erythematosus (SLE). Neopterin levels can be used as prognostic predictors for certain types of malignancies. Measurement of neopterin levels has particular value for monitoring patients infected with HIV. Neopterin is eliminated primarily in the urine, so evaluation of urinary neopterin levels may be useful in assessing activation of the cellular immunity system even in the absence of typical clinical symptoms, since a correlation has been observed with the course of diseases involving cellular immunity activation and urinary levels.

SKU: USN1060-49 Categories: , ,

Description

Principle of the Test: Neopterin ELISA kit is based on competitive binding of human Neopterin from serum samples and enzyme-labeled Neopterin to Neopterin specific antibodies immobilized on microtiter plates. After a washing step, chromogenic substrate is added and colour developed. The enzymatic reaction (blue colour is inversely proportional to the amount of Neopterin present in the sample. The reaction is terminated by adding stopping solution (converts blue to yellow. Absorbance is then measured on an ELISA reader at 450nm and the concentration of Neopterin in samples and control is read off the standard curve.

Additional information

Range

Intra-assay Precision CV: < 10% for Neopterin range 2-50nmol/L
Inter-assay Precision CV: < 10% for Neopterin range 2-50nmol/L

Sensitivity

0.7nmol/L

Kit Components

N1060-49A: Micrototer Plate, 1×96 wells
N1060-49B: Standard 0nM, 1×1.5ml
N1060-49C: Standard 1.35nM, 1×1.5ml
N1060-49D: Standard 4nM, 1×1.5ml
N1060-49E: Standard 12nM, 1×1.5ml
N1060-49F: Standard 37nM, 1×1.5ml
N1060-49G: Standard 111nM, 1×1.5ml
N1060-49H: Assay Buffer, 1x21ml
N1060-49J: Control 1, 4-8.3nM, 1×1.5ml
N1060-49K: Control 2, 15.2-282nM, 1×1.5ml
N1060-49L: Neopterin (HRP Conjugate, 1x13ml
N1060-49M: Neopterin Antibody, 1x5ml
N1060-49N: Wash Buffer, 20X, 1x50ml
N1060-49P: TMB Substrate Solution, 1x19ml
N1060-49Q: Stop Solution, 1N H2SO4, 1x19ml

Storage and Stability

Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.