N-Terminal Pro Brain Natriuretic Peptide (NT-ProBNP) BioAssay ELISA Kit (Mouse)

£1,004.00 96 Tests

N-Terminal Pro Brain Natriuretic Peptide (NT-ProBNP) BioAssay ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NT-ProBNP in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

SKU: US027010 Categories: , ,

Additional information

Detection Range

6.9-5000pg/ml

Sensitivity

2.5pg/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components

*027010A: Microtiter Strips, 1×48 wells or 1×96 wells
*027010B: Standard, 2×1 vial
027010C: Standard Diluent, 1x10ml or 1x20ml
*027010D: Detection Reagent A (green), 1x60ul or 1x120ul
*027010E: Detection Reagent B (red), 1x60ul or 1x120ul
027010F: Assay Diluent A, 1x6ml or 1x12ml
027010G: Assay Diluent B, 1x6ml or 1x12ml
027010H: TMB Substrate, 1×4.5ml or 1x9ml
*027010J: Positive Control, 215.6ng/ml, 1×1 vial
027010K: Stop Solution, 1x3ml or 1x6ml
027010L: Wash Buffer, 30X, 1x10ml or 1x20ml

Precaution

The Stop Solution (027010K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *027010A, *027010B, *027010D, *027010E and *027010J at -20?C. After reconstitution of *027010J, store at 4?C and use within 5 days. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37?C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen

Mouse

Shelf Life

6 Months