Motilin (MTL) BioAssay ELISA Kit (Human)

£960.00 96 Tests

Motilin (MTL) BioAssay ELISA Kit (Human) is a competitive inhibition immunoassay for in vitro quantitative measurement of MTL in human serum, plasma and other biological fluids.

SKU: US026884 Categories: , ,

Additional information

Detection Range

74.07-6000pg/ml

Sensitivity

29.64pg/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components

*026884A: Microtiter Strips, 1×96 wells
*026884B: Standard, 2×1 vial
026884C: Standard Diluent, 1x20ml
*026884D: Detection Reagent A (green), 1x120ul
*026884E: Detection Reagent B (red), 1x120ul
026884F: Assay Diluent A, 1x12ml
026884G: Assay Diluent B, 1x12ml
026884H: TMB Substrate, 1x9ml
026884K: Stop Solution, 1x6ml
026884L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (026884K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *026884A, *026884B, *026884D and *026884E at -20?C. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37?C.
3. Aspirate and wash 3 times.
4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
5. Aspirate and wash 5 times.
6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37?C.
7. Add 50ul Stop Solution. Read absorbance at 450nm immediately.

Source Antigen

Human

Specificity

Human

Shelf Life

6 Months