Monocyte Chemotactic Protein 2 (MCP2) BioAssay ELISA Kit (Canine)

£1,036.00 96 Tests

Monocyte Chemotactic Protein 2 (MCP2) BioAssay ELISA Kit (Canine) is a sandwich enzyme immunoassay for the in vitro quantitative measurement of MCP2 in canine serum, plasma, tissue homogenates and other biological fluids.

Additional information

Detection Range

31.2-2000pg/ml

Sensitivity

11.7pg/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Test Principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to MCP2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MCP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MCP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of MCP2 in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Kit Components

*026860A: Microtiter Strips, 1×96 wells, Pre-coated, ready to use
*026860B: Standard, 2x1vial
026860C: Standard Diluent, 1x20ml
*026860D: Detection Reagent A, 1x120ul
*026860E: Detection Reagent B, 1x120ul
026860F: Assay Diluent A, 1x12ml
026860G: Assay Diluent B, 1x12ml
026860H: TMB Substrate, 1x9ml
026860K: Stop Solution, 1x6ml
026860L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (026860K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *026860A, *026860B, *026860D and *026860E at -20?C. Store all the other components at 4?C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37?C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen

Canine

Shelf Life

6 Months