Monocyte Chemotactic Protein 1 (MCP1) BioAssay ELISA Kit (Rat)

£1,007.00 96 Tests

Monocyte Chemotactic Protein 1 (MCP1) BioAssay ELISA Kit (Rat) is a sandwich enzyme immunoassay for in vitro quantitative measurement of MCP1 in rat serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.

Additional information

Detection Range

0.156-10ng/ml

Sensitivity

0.061ng/ml

Precision

Intra-Assay:
CV < 10%

Inter-Assay:
CV < 12%

Kit Components

*026853A: Microtiter Plate, 1×96 wells, Pre-coated, ready to use
*026853B: Standard, 2×1 vial?
026853C: Standard Diluent, 1x20ml
*026853D: Detection Reagent A (100X), 1x120ul?
*026853E: Detection Reagent B (100X), 1x120ul?
026853F: Assay Diluent A (2X), 1x6ml
026853G: Assay Diluent B (2X), 1x6ml
026853H: TMB Substrate, 1x9ml
*026853J: Positive Control, 99.3pg/ml, 1×1 vial
026853K: Stop Solution, 1x6ml
026853L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (026853K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *026853A, *026853B, *026853D, *026853E and *026853J at -20?C. After reconstitution of *026853J, store at 4?C and use within 5 days. Store all the other components at 4?C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards;
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37?C.
3. Add 100ul working Detection Reagent A immediately. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul working Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen

Rat

Specificity

Rat

Shelf Life

6 Months