Why might it be a problem to use serum that is made from donors who have received a blood transfusion?

HLA antibodies are not naturally occurring and can only be acquired by pregnancy, transfusion of blood products or transplantation. Therefore, for a donor pool of un-transfused male donors with blood group AB, provided the donors are healthy, you can expect a very low incidence of HLA antibodies.
Depending on your specific application, you would need to use serum from non-transfused patients if it is important that the serum you receive is HLA free.
Also see question: Can donors who have received blood transfusions, donate blood for Human AB Serum collection purposes?

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