Melatonin Sulfate, Human (6-Hydroxymelatonin Sulfate, 6-Sulfatoxymelatonin) BioAssay ELISA Kit

£1,065.00 96 Tests

Test Principle:
Solid phase enzyme-linked immunosorbent assay (ELISA) based on the competition principle. An unknown amount of antigen present in the sample and a fixed amount of enzyme labelled antigen compete for the binding sites of the antibodies coated onto the wells. After incubation the wells are washed to stop the competition reaction. After the substrate reaction the intensity of the developed color is inversely proportional to the amount of the antigen in the sample. Results of samples can be determined directly using the standard curve.

SKU: USM2871-22H Categories: , ,

Additional information

Intended Use

Enzyme immunoassay for the quantitative determination of melatonin sulfate in human urine.

Sample Test

Urine

Kit Components

M2871-22H1: Microtiter Plate, 1×96 wells
M2871-22H2: PAB, 1x200ul
M2871-22H3: Melatonin Sulfate (HRP), 40x, 1x200ul
M2871-22H4: Standard, 0ng/ml, 1x100ul
M2871-22H5: Standard, 1.7ng/ml, 1x100ul
M2871-22H6: Standard, 5.2ng/ml, 1x100ul
M2871-22H7: Standard, 15.6ng/ml, 1x100ul
M2871-22H8: Standard, 46.7ng/ml, 1x100ul
M2871-22H9: Standard, 140ng/ml, 1x100ul
M2871-22H10: Standard, 420ng/ml, 1x100ul
M2871-22H11: Low Control 1, 1x100ul
H2871-22H12: High Control 2, 1x100ul
H2871-22H13: Assay Buffer, 1x60ml
H2871-22H14: Wash Buffer, 20x, 1x50ml
H2871-22H15: TMB Substrate, 1x12ml
H2871-22H16: Stop Solution, 1M H2SO4, 1x12ml

Storage and Stability

Store all components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Shipping Temperature

On Dry Ice

Source Antigen

Human

Shelf Life

6 Months