Mca-SEVNLDAEFR-K(Dnp)-RR-NH2
£125.00 1mg
Please note: this product can only be supplied to registered research and development facilities, and may be used for in vitro research use only, not for administration to humans or animals.
FRET substrate for beta-secretase 1 (BACE-1).
Mca-SEVNLDAEFR-K(Dnp)-RR-NH2, also knowm as Mca-(Asn ¶ · °,Leu ¶ · ¹)-Amyloid ?/A4 Protein Precursor ‚‡ ‚‡ ‚ (667-676)-Lys(Dnp)-Arg-Arg amide, is a Beta-secretase 1 (BACE-1) peptide FRET substrate, containing the ‘Swedish’ Lys-Met/Asn-Leu mutation of the amyloid precursor protein (APP) ?-secretase cleavage site. Cleavage at -Leu-Asp- of Mca-SEVNLDAEFR-K(Dnp)-RR-NH2 liberates the highly fluorescent 7-methoxycoumarin (Mca) fragment from the proximity quenching effect of the 2,4-dinitrophenyl (Dnp) internal quencher resulting in a large and easily detectable increase in fluorescence intensity.
Please contact us for availability.
Additional information
| Other Names | Mca-(Asn ¶ · °,Leu ¶ · ¹)-Amyloid ?/A4 Protein Precursor ‚‡ ‚‡ ‚ (667-676)-Lys(Dnp)-Arg-Arg amide |
|---|---|
| Three Letter Sequence | Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(Dnp)-Arg-Arg-NH2 |
| Molecular Weight | 2001.1 |
| Molecular Formula | C86H125N27O29 |
| Sequence | Mca-SEVNLDAEFR-K(Dnp)-RR-NH2 |
| Solubility | Soluble in DMSO |
| Appearance | Freeze dried solid |
| Storage | Store desiccated, frozen and in the dark |
| Purity | >95% by HPLC |
| Modifications | 7-methoxycoumarin (Mca) at N terminal, 2,4-dintrophenyl on lysine 11, C terminal amide |
| Searchable Words | Mca-SEVNLDAEFR-K(Dnp)-RR-NH2, 438625-61-7, SEVNLDAEFRKRR, Mca-(Asn ¶ · °,Leu ¶ · ¹)-Amyloid ?/A4 Protein Precursor ‚‡ ‚‡ ‚ (667-676)-Lys(Dnp)-Arg-Arg amide, Beta-secretase 1, BACE-1, FRET substrate, Swedish Lys-Met/Asn-Leu mutation, fluorescent 7-methoxycoumarin, PS-020, PS020 |