Mca-SEVNLDAEFR-K(Dnp)-RR-NH2
£125.00 1mg
FRET substrate for beta-secretase 1 (BACE-1).
Mca-SEVNLDAEFR-K(Dnp)-RR-NH2, also knowm as Mca-(Asn ¶ · °,Leu ¶ · ¹)-Amyloid ?/A4 Protein Precursor ‚‡ ‚‡ ‚ (667-676)-Lys(Dnp)-Arg-Arg amide, is a Beta-secretase 1 (BACE-1) peptide FRET substrate, containing the ‘Swedish’ Lys-Met/Asn-Leu mutation of the amyloid precursor protein (APP) ?-secretase cleavage site. Cleavage at -Leu-Asp- of Mca-SEVNLDAEFR-K(Dnp)-RR-NH2 liberates the highly fluorescent 7-methoxycoumarin (Mca) fragment from the proximity quenching effect of the 2,4-dinitrophenyl (Dnp) internal quencher resulting in a large and easily detectable increase in fluorescence intensity.
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Additional information
| Other Names | Mca-(Asn ¶ · °,Leu ¶ · ¹)-Amyloid ?/A4 Protein Precursor ‚‡ ‚‡ ‚ (667-676)-Lys(Dnp)-Arg-Arg amide |
|---|---|
| Three Letter Sequence | Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(Dnp)-Arg-Arg-NH2 |
| Molecular Weight | 2001.1 |
| Molecular Formula | C86H125N27O29 |
| Sequence | Mca-SEVNLDAEFR-K(Dnp)-RR-NH2 |
| Solubility | Soluble in DMSO |
| Appearance | Freeze dried solid |
| Storage | Store desiccated, frozen and in the dark |
| Purity | >95% by HPLC |
| Modifications | 7-methoxycoumarin (Mca) at N terminal, 2,4-dintrophenyl on lysine 11, C terminal amide |
| Searchable Words | Mca-SEVNLDAEFR-K(Dnp)-RR-NH2, 438625-61-7, SEVNLDAEFRKRR, Mca-(Asn ¶ · °,Leu ¶ · ¹)-Amyloid ?/A4 Protein Precursor ‚‡ ‚‡ ‚ (667-676)-Lys(Dnp)-Arg-Arg amide, Beta-secretase 1, BACE-1, FRET substrate, Swedish Lys-Met/Asn-Leu mutation, fluorescent 7-methoxycoumarin, PS-020, PS020 |