MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2

£175.00 1mg

SKU: CV-070 Categories: ,

FRET substrate for the severe acute respiratory syndrome coronavirus main protease (SARS-CoV Mpro).

MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 is a FRET substrate for the severe acute respiratory syndrome coronavirus main protease (SARS-CoV Mpro). The MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 substrate sequence is derived from residues P4 ?P5′ of the SARS-CoV Mpro N-terminal autoprocessing site which has the sequence AVLQSGFRK. Enzmatic cleavage between Q4 and S5 of MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 releases the highly fluorescent 7-methoxycoumarin (Mca) group from the influence of the 2,4-dinitrophenyl (Dnp) internal quencher, resulting in a large increase in fluorescence intensity.

Additional information

Other Names

SARS Main Protease FRET Substrate

Three Letter Sequence

Mca-Ala-Val-Leu-Gln-Ser-Gly-Phe-Arg-Lys(Dnp)-Lys-NH2

Molecular Weight

1514.7

Molecular Formula

C69H99N19O20

Sequence

Mca-AVLQSGFRK(Dnp)K-NH2

Solubility

Soluble as a stock solution in DMSO

Appearance

Freeze dried solid

Storage

Store desiccated, frozen and in the dark

Purity

>95% by HPLC

Modifications

N terminal 7-methoxycoumarin (Mca), C terminal amide, lysine 9 has 2,4-dinitrophenyl (Dnp) attached

Searchable Words

MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2, FRET substrate, severe acute respiratory syndrome coronavirus main protease, SARS-CoV Mpro, AVLQSGFRK, 7-methoxycoumarin, Mca, 2,4-dinitrophenyl, fluorescence, cv-070, cv070, 932391-88-3, covid, covid-19, covid 19