MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2
£175.00 1mg
Please note: this product can only be supplied to registered research and development facilities, and may be used for in vitro research use only, not for administration to humans or animals.
FRET substrate for the severe acute respiratory syndrome coronavirus main protease (SARS-CoV Mpro).
MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 is a FRET substrate for the severe acute respiratory syndrome coronavirus main protease (SARS-CoV Mpro). The MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 substrate sequence is derived from residues P4 ?P5′ of the SARS-CoV Mpro N-terminal autoprocessing site which has the sequence AVLQSGFRK. Enzmatic cleavage between Q4 and S5 of MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 releases the highly fluorescent 7-methoxycoumarin (Mca) group from the influence of the 2,4-dinitrophenyl (Dnp) internal quencher, resulting in a large increase in fluorescence intensity.
Additional information
| Other Names | SARS Main Protease FRET Substrate |
|---|---|
| Three Letter Sequence | Mca-Ala-Val-Leu-Gln-Ser-Gly-Phe-Arg-Lys(Dnp)-Lys-NH2 |
| Molecular Weight | 1514.7 |
| Molecular Formula | C69H99N19O20 |
| Sequence | Mca-AVLQSGFRK(Dnp)K-NH2 |
| Solubility | Soluble as a stock solution in DMSO |
| Appearance | Freeze dried solid |
| Storage | Store desiccated, frozen and in the dark |
| Purity | >95% by HPLC |
| Modifications | N terminal 7-methoxycoumarin (Mca), C terminal amide, lysine 9 has 2,4-dinitrophenyl (Dnp) attached |
| Searchable Words | MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2, FRET substrate, severe acute respiratory syndrome coronavirus main protease, SARS-CoV Mpro, AVLQSGFRK, 7-methoxycoumarin, Mca, 2,4-dinitrophenyl, fluorescence, cv-070, cv070, 932391-88-3, covid, covid-19, covid 19 |