Matrix Metalloproteinase 13 (MMP13) BioAssay ELISA Kit (Bovine)

£1,068.00 96 Tests

Matrix Metalloproteinase 13 (MMP13) BioAssayTM ELISA Kit (Bovine) is a sandwich enzyme immunoassay for in vitro quantitative measurement of MMP13 in bovine serum, plasma, tissue homogenates, cell lysates and other biological fluids.

SKU: US026712 Categories: , , ,

Additional information

Detection Range

1.56-100ng/ml

Sensitivity

0.59ng/ml

Precision

Intra-Assay:
CV <10%

Inter-Assay:
CV <12%

Kit Components

*152433A: Microtiter Strips, 1×96 wells, Pre-coated, ready to use
*152433B: Standard, 2×1 vial
152433C: Standard Diluent, 1x20ml
*026712D: Detection Reagent A (green), 1x120ul
*026712E: Detection Reagent B (red), 1x120ul
152433F: Assay Diluent A, 1x12ml 152433G: Assay Diluent B, 1x12ml 152433H: TMB Substrate, 1x9ml 152433J: Positive Control, 19.73ng/ml, 1×1 vial
152433K: Stop Solution, 1x6ml 026712L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (026712K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Notes on 026712J

Positive Control

Intended Use

The control vial is from a bovine serum sample known to contain MMP13. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.

Characteristics

The control vial contains porcine MMP13 in bovine serum (lyophilized powder). Note: The bovine source material used in this product are from healthy animal of SPF standard (specific pathogen-free animals).

Expected Values

19.73ng/ml

Usage

Dissolve with 150ul standard dilutent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20?C. After reconstitution, store Positive Control vial at 4?C and use within 5 days.

Storage and Stability

Store *026712A, *026712B, *026712D, *026712E and *026712J at -20?C. After reconstitution of *026712J, store at 4?C and use within 5 days. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37?C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen

Bovine

Specificity

Bovine

Shelf Life

6 Months