M Broth (Powder)

£466.56 500g

Intended Use:
M Broth is used for the cultivation of Salmonella spp in a laboratory setting. M-Broth is not intended for use in the diagnosis of disease or other conditions in humans.

Description:
M-Broth is prepared according to the formula of Sperber and Diebel, and contains the nutrients necessary for good growth and flagella development of Salmonella. This medium is used for cultivating Salmonella in foods and feeds by the accelerated enrichment serology (ES) procedure. M-Broth conforms to standard procedures recommended in food testing and monoclonal and polyclonal enzyme immunoassays (EIA).

Directions per Liter:
1. Dissolve 36.2g of the medium in one liter of purified water.
2. Mix thoroughly.
3. Autoclave at 121?C for 15 minutes.

Quality Control Specifications:
Dehydrated Appearance: Powder is homogeneous with small lumps and light beige.
Prepared Appearance: Prepared medium is yellow to amber and clear to trace hazy with no to light precipitate.
Expected Cultural Response: Cultural response in M-Broth at 35 ? 2?C and examined for growth after 18 – 24hours incubation.

Typical Formulation:
Enzymatic Digest of Casein 12.5g/L
Yeast Extract 5.0g/L
D-Mannose 2.0g/L
Sodium Citrate 5.0g/L
Sodium Chloride 5.0g/L
Potassium Phosphate 5.0g/L
Manganese Chloride 0.14g/L
Magnesium Sulfate 0.8g/L
Ferrous Sulfate 0.04g/L
Polysorbate 80 0.75g/L

Final pH:
7.0 ? 0.2 at 25?C

Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4?C and used within a short period of time.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Please contact us for availability.

SKU: US-M2500 Categories: ,