Isoprostane (8-iso-PGF2a) Urine ELISA Kit, BioAssay

£765.00 1 Kit

Isoprostanes are prostaglandin-like compounds that are produced by free radical mediated peroxidation of lipoproteins. This kit is for the quantification of 15-isoprostane F2t (also known as 8-epi-PGF2a or 8-iso-PGF2a) in urine samples. Levels of 15-isoprostane F2t in urine are useful for the non-invasive assessment of oxidant stress in vivo. 15-isoprostane F2t has also been shown to be a potent vasoconstrictor in rat kidneys and rabbit lungs, and plays a causative role in atherogenesis. Elevated isoprostane levels are associated with hepatorenal syndrome, rheumatoid arthritis, atherosclerosis, and carcinogenesis.

Principle of the Assay:
This kit is a competitive ELISA for determining levels of 15-isoprostane F2t (the best characterized isoprostane) in urine samples. Briefly, urine samples are mixed with an enhanced dilution buffer that essentially eliminates interference due to non-specific binding. The 15-isoprostane F2t in the samples or standards competes with 15-isoprostane F2t conjugated to HRP for binding to a polyclonal antibody specific for 15-isoprostane F2t coated on the microplate. The HRP activity results in colour development when substrate is added, with the intensity of the colour proportional to the amount of 15-isoprostane F2t bound and inversely proportional to the amount of unconjugated 15-isoprostane F2t in the samples or standards.

SKU: USI9055 Categories: , ,

Additional information

Detection Range

0.05-100ng/ml

Kit Components

I9055-01: Microtiter Strip precoated with 15-isoprostane F2t, 1x96wells
I9055-02: 15-isoprostane F2t Standard (1ug/ml), 2x60ul
I9055-03: Enhanced Dilution Buffer 1x100ml
I9055-04: Wash Buffer, 5X, 1x40ml
I9055-05: TMB Substrate, 1x25ml
I9055-06: 15-Isoprostane F2t (HRP), 1x250ul
I9055-07: b-Glucuronidase, 2x100ul

Storage and Stability

Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Temperature

On Dry Ice

Shelf Life

6 Months