Islet Amyloid Polypeptide (IAPP) BioAssay ELISA Kit (Rat)

£1,007.00 96 Tests

Islet Amyloid Polypeptide (IAPP) BioAssay ELISA Kit (Rat) is a competitive inhibition immunoassay for in vitro quantitative measurement of IAPP in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

SKU: US026301 Categories: ,

Additional information

Detection Range

6.17-500pg/ml

Sensitivity

2.78pg/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components

*026301A: Microtiter Strips, 1×96 wells, Pre-coated, ready to use
*026301B: Standard, 2×1 vial
026301C: Standard Diluent, 1x20ml
*026301D: Detection Reagent A (green), 1x1vial
*026301E: Detection Reagent B (red), 1x120ul
026301F: Assay Diluent A, 1x12ml
026301G: Assay Diluent B, 1x12ml
026301H: TMB Substrate, 1x9ml
026301K: Stop Solution, 1x6ml
026301L: Wash Buffer, 30X, 1x20ml
026301M: Detection Reagent A Diluent, 1x300ul

Precaution

The Stop Solution (026301K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *026301A, *026301B, *026301D and *026301E at -20?C. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37?C.
3. Aspirate and wash 3 times.
4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
5. Aspirate and wash 5 times.
6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37?C.
7. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen

Rat

Shelf Life

6 Months