Interleukin 8 (IL8) BioAssay ELISA Kit (Human)

£815.00 96 Tests

Interleukin 8 (IL8) BioAssay ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL8 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Additional information

Detection Range

15.6-1000pg/ml

Sensitivity

6.8pg/ml

Precision

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components

*026272A: Microtiter Strips, 1×96 wells, Pre-coated, ready to use
*026272B: Standard, 2x1vial
026272C: Standard Diluent, 1x20ml
*026272D: Detection Reagent A (green), 1x120ul
*026272E: Detection Reagent B (red), 1x120ul
026272F: Assay Diluent A, 1x12ml
026272G: Assay Diluent B, 1x12ml
025266H: TMB Substrate, 1x9ml
*026272J: Positive Control, 340.1pg/ml, 1x1vial
026272K: Stop Solution, 1x6ml
026272L: Wash Buffer, 30X, 1x20ml

Precaution

The Stop Solution (026272K) included for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability

Store *026272A, *026272B, *026272D, *026272E and *026272J at -20?C. After reconstitution of *026272J, store at 4?C and use within 5 days. Store all the other components at 4?C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Notes on 026272J

Positive Control

Intended Use

The Positive Control is from a human serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.

Characteristics

The positive control vial contains human IL8 in human serum (lyophilized powder). Note: The human based material used in this product was screened for HIV, Hepatitis A, B, C, HTLV and Syphilis.

Expected Values

340.1pg/ml

Usage

Dissolve with 150ul standard dilutent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20?C. After reconstitution, store Positive Control vial at 4?C and use within 5 days.

Assay Procedure Summary

1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37?C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37?C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37?C.
6. Aspirate and wash 5 times.
7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37?C.
8. Add 50ul Stop Solution. Read at 450nm immediately.

Source Antigen

Human

Shelf Life

6 Months